Aim and objective To evaluate and compare the microleakage of unmodified microhybrid composite and 0.2% chitosan-incorporated composite in class V cavities restored immediately and after 3 months of storage in artificial saliva. Materials and methods Sixty human permanent maxillary premolars were collected and standardized class V cavity prepared on the buccal surface of each tooth with dimensions: mesiodistally 3 mm, occluso cervically 2 mm, and depth of 1.5 mm and restored with microhybrid composite and chitosan-incorporated composite resins respectively and randomly divided: Group I: control-microhybrid composite ( n = 30): (a) 15 teeth tested immediately (b) 15 teeth tested after 3 months. Group II–restored with chitosan + composite ( n = 30): (a) 15 teeth tested immediately (b) 15 teeth tested after 3 months. Specimens were stored in artificial saliva following which a dye extraction test was carried out using a spectrophotometer. Results There was no statistically significant difference in microleakage score between the chitosan-composite group and unmodified composite group when evaluated immediately after placing the restoration. Microleakage values of the unmodified composite group increased significantly after 3 months of storage in artificial saliva and values of the chitosan-composite group did not differ significantly even after 3 months of storage. Microleakage was seen significantly less in the chitosan-composite group compared to the unmodified composite group after 3 months of storage in artificial saliva. Conclusion It can be concluded that chitosan-incorporated composite seems to have improved mechanical properties and forms a more stable bond when compared with unmodified microhybrid composite in addition to being antibacterial. Clinical significance Considering the advantageous properties of this material, it may be clinically useful in restoring class V cavities in patients with high caries risk. However, further in vitro and in vivo studies need to be carried out. How to cite this article Deb A, Pai V, Nadig RR. Evaluation of Immediate and Delayed Microleakage of Class V Cavities Restored with Chitosan-incorporated Composite Resins: An In Vitro Study. Int J Clin Pediatr Dent 2021;14(5):621–627.

Aim: To evaluate the in-vitro microleakage of traditional micro hybrid composite resin and 0.2% chitosan-incorporated composite resin when restored in Class V cavities using total etch versus self-etch adhesives after storing in artificial saliva for 24 h. Materials and Methodology: Sixty permanent maxillary premolars collected and Class V cavities were prepared on buccal surface of each tooth (dimensions: mesio-distally 3 mm, occluso cervically 2 mm, and depth of 1.5 mm) and restored with Group 1: micro hybrid (30 teeth) and Group 2: chitosan-incorporated composite (30 teeth), which was further subdivided into: (a) 15 teeth using total-etch adhesives. (b) 15 teeth using self-etch adhesives. Next dye extraction test was carried out using spectrophotometer. Results: Comparison within groups: In Group 1: Self-etch demonstrated less microleakage (0.0129) compared with total etch (0.0183). The difference was statistically significant, and in Group 2: No statistically significant difference was found in mean microleakage scores after using either self-etch (0.0118) or total etch adhesives (0.0120). Conclusion: It can be concluded that chitosan-incorporated composite seems to have improved mechanical properties with a stable bond when used with either self-etch or total etch adhesives in addition to being antibacterial. It may be clinically useful in restoring Class V cavities in patients with high caries risk. However, further in vitro and in-vivo studies need to be carried out.