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Introduction: Vesicobullous disorders represent a heterogenous group of dermatoses with protean manifestations in which the primary lesion is a vesicle or a bulla on the skin or mucous membrane or both. These lesions can be extremely debilitating, may have serious sequelae, and even fatal, necessating early treatment and intervention to prevent further morbidity and mortality. Many of these blistering diseases mimic each other clinically, Histopathology together with immunofluorescence are used for diagnosing vesiculobullous lesions of skin. Materials and Methods: The present study is done at out institute for a period of 3 years from 2016 to 2019 All the vesiculo bullous lesions which we received during this period have been included in our study. We received a total of 42 cases during our study period. Light microscopic examination followed by DIF was done for definitive diagnosis. The pattern and distribution of immune complex deposits was analysed under fluorescence microscope and correlated with histological findings Results: Bullous pemphigoid was the common vesiculobullous disease (38.09%) followed by pemphigus vulgaris. dif showed linear type of immune deposits in epidermal basement membrane zone in bullous pemphigoid and in pemphigus vulgaris intercellular lace like deposition of immune complexes was seen. Conclusion:Vesiculobullous disorders represent a heterogeneous group of dermatoses Punch biopsy of the skin is a simple, inexpensive, safe OPD procedure, causing minimal discomfort lesions and DIF studies are an adjunct to the histomorphology. DIF which is the gold standard and sensitive test in making a definitive diagnosis aids in distinguishing non immune lesions from immune mediated lesions which pose a diagnostic dilemma both clinically and histologically An integrated approach of clinical findings in conjunct with histomorphology and DIF assist in accurate diagnosis and proper patient management.

A 20 year old female presented with a recurrent soft tissue swelling over the medial aspect of proximal phalanx of left little finger. It had recurred one year after excision. There was no lymphadenopathy or bony involvement. Previous histopathology reports were not available. After excision histopathological diagnosis was aggressive digital papillary adenocarcinoma. Later Ray's amputation of little finger was planned. Aggressive digital papillary adenocarcinomas are rare sweat gland tumors which occur on hands, fingers, and toes. They have high local recurrence rate and may metastasize to lungs and lymph nodes.

INTRODUCTION:The cytologic examination of body fluids is a common practice and of distinct value in confirming or disapproving malignant metastatic tumors to the body cavities. It also gives information on specific pathologic processes as tuberculous or otherwise. AIMS AND OBJECTIVES: The present study was carried out to compare the sediments obtained by ordinary centrifuge and Cytospin II centrifuge using pleural and ascitic fluids as specimen samples. The emphasis was on cell yield and distribution, preservation of cell morphology, type of malignant cells and comparing the results for cases which were positive by both methods. MATERIALS AND METHODS: A total of 150 random samples (57 pleural and 93 peritoneal fluids) were studied over a period of one year and two months. The samples were split and processed by both methods simultaneously and results were compared. Histopathological correlation was done wherever possible. RESULTS: There were 25 cases (16%) of malignant effusions, 9(36%) from pleural and 16(64%) from peritoneal cavities. The most common primary site in female patients was ovary, whereas, in male patients it was the gastrointestinal tract. All 25 cases (100%) of malignancy were detected by Cytospin II method while the ordinary centrifuge picked up only 23 cases (92%). CONCLUSIONS:The comparative study between the Cytospin II and ordinary centrifuge revealed that the Cytospin II preparation gives a better yield of cells specially when they are scanty, morphology of cells is very well preserved and much time is saved in screening the slide. The Cytospin II is better in picking up malignant cells than the ordinary centrifuge.

INTRODUCTION:The present comparative study of PAP and REAP stain was carried out to assess the quality of staining, duration of staining, cost effectiveness and preservation of staining. MATERIALS AND METHODS:A total of 200 samples were studied over a period of 2 years. The pap smears were obtained from the obstetrics and gynecology out-patient department, fixed in 95% ethyl alcohol and stained simultaneously by both the methods in the department of cytology. RESULTS: Pap stain showed 2 cases (1%) of sub optimal cytoplasmic and nuclear staining whereas REAP stain showed 6 cases (3%) of suboptimal cytoplasmic staining and 4 cases (2%) of suboptimal nuclear staining. A batch of ten slides each was stained by conventional PAP method and REAP method and the time taken was 35 min and 7 min respectively. The cost per slide worked out to be Rs. 40 in case of conventional PAP method and Rs. 10 in case of REAP method. Slides stained by both methods showed excellent preservation for 2 years. CONCLUSIONS: The REAP staining method for study of cervical smears in cancer screening programmes is a simple and technician friendly protocol with minimum use of alcohol, that does not compromise on staining quality and diagnostic standards. It can be easily adapted as a viable alternative to conventional PAP method which is time consuming and expensive for mass screening of cervical cancer in limited resource setting like India. We recommend this procedure for all mass cervical cancer screening programmes.