Comparative Study of Body Fluid Cytology Using Cytospin - Ii and Ordinary Centrifuge

Abstract

INTRODUCTION:The cytologic examination of body fluids is a common practice and of distinct value in confirming or disapproving malignant metastatic tumors to the body cavities. It also gives information on specific pathologic processes as tuberculous or otherwise. AIMS AND OBJECTIVES: The present study was carried out to compare the sediments obtained by ordinary centrifuge and Cytospin II centrifuge using pleural and ascitic fluids as specimen samples. The emphasis was on cell yield and distribution, preservation of cell morphology, type of malignant cells and comparing the results for cases which were positive by both methods. MATERIALS AND METHODS: A total of 150 random samples (57 pleural and 93 peritoneal fluids) were studied over a period of one year and two months. The samples were split and processed by both methods simultaneously and results were compared. Histopathological correlation was done wherever possible. RESULTS: There were 25 cases (16%) of malignant effusions, 9(36%) from pleural and 16(64%) from peritoneal cavities. The most common primary site in female patients was ovary, whereas, in male patients it was the gastrointestinal tract. All 25 cases (100%) of malignancy were detected by Cytospin II method while the ordinary centrifuge picked up only 23 cases (92%). CONCLUSIONS:The comparative study between the Cytospin II and ordinary centrifuge revealed that the Cytospin II preparation gives a better yield of cells specially when they are scanty, morphology of cells is very well preserved and much time is saved in screening the slide. The Cytospin II is better in picking up malignant cells than the ordinary centrifuge.

Cytological study of body fluids for malignancy

“…Since mesothelial and synovial tumors are rare, this method is useful to detect metastatic malignant cells to the body cavities. The method is more of prognostic value rather than for the early detection or prevention of further tumor growth [2].…”

“…The clinical diagnosis noted in the present study slightly differed from a study by Deshpande AK. et al [2] from Hyderabad where, authors encounter 12 cases (8%) of tuberculous effusions, 8 (66.6%) from pleural and 4(33.33%) from peritoneal cavities.…”

“…The cytologic examination of body fluids is of distinct value in confirming or disapproving malignant metastatic tumors to the cavities [2]. The main serosal body cavity comprises pleural, peritoneal, pericardial, synovial and cerebrospinal fluids.…”

Comparative study of body fluid cytology using cytocentrifuge and ordinary centrifuge

“…Comparable findings regarding the diseases present in the fluids have been documented by Vidushi et al 4 in which 100 samples were studied, out of which 05 were positive for malignancy and 95 were benign. In the study done by Deshpande et al 10 in which 150 samples were studied, out of which 25 cases being positive for malignant cells and 125 being benign. In the study done by Joshi et al, 2 out of 150 samples, 34 were positive for malignancy and 116 were benign.…”

Rapid Fixation –Is it an Effective Method in the Diagnosis of Cytological Smears

“…The material from body cavities and effusions like urine, pleura, ascitic fluid, pericardial, peritoneal wash and gastric washings and Put these samples in clean air tight centrifuged tube. Rotate the tube at 1500 rounds per minute (RPM) for 5 minutes in Cytocentrifugation (REMI R-8C) [4]. Discard the supernatant fluid .Make multiple smears from the sediments on a clean glass slide.…”