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Background:The development of health care information technology and the emergence of artificial intelligence have yielded tools to improve the quality of several health care processes. Aim: to assess the health care providers" perception about artificial intelligence applications at Main Assiut University Hospital. Study design: A descriptive survey design was used. Setting: This study was conducted at Main Assuit University Hospital. Subjects: the study subjects included three types of health care providers (no=763): physicians (no=202), nurses (no=535), and technicians (no=26). Data collection tool: The study data collected through selfadministered questionnaire which consists of personal characteristics data and perception about artificial intelligence questionnaire. Results: The overall perception of studied healthcare providers about artificial intelligence applications was moderate. The highest percentage of studied Physicians had a high level of perception about artificial intelligence applications. The highest percentage of studied Nurses had a moderate level of perception. The highest percentage of studied Technicians had an equal percent of both high and moderate level of perception. Conclusions: There were highly statistical significant differences between the perception levels about artificial intelligence applications with job type and educational qualification. There were no statistical significant differences between the perception levels about artificial intelligence applications with age, gender, and years of experience. Recommendations: Convey workshops and training programs for health care providers to educate them about artificial intelligence potential applications and synergies & preparing and distributing a manual about artificial intelligence among health care providers.