Background: Primary infertility and secondary infertility of men in the reproductive age represent 1.9 and 10.5%, respectively. Many etiological factors are involved, among which urogenital bacterial infections play an important role. Materials and Methods:Semen analysis, bacteriological culture, and sensitivity analyses were carried out to investigate the effect of genitourinary infections on semen parameters of infertile men. Results: Staphylococcus aureus was the most common isolated pathogen (46.2%) followed by urogenic gram-negative pathogens (24.1%). The isolated microorganisms are highly sensitive to piperacillin/tazobactam, imipenem, meropenem, gentamicin, doxycycline, amikacin, and nitrofurantoin. These antibiotics could be used empirically while awaiting the results of semen culture. Conclusion: Semen culture is an important diagnostic tool in all patients undergoing fertility investigations to detect genitourinary infections, pyospermia, and bacteriospermia. Moreover, early treatment should be considered according to the results of culture whenever possible. Wide range of broad-spectrum antibiotics can be used as an empirical treatment for infertile patients to adjust the seminal parameters and reduce the number of leukocytes in semen ejaculates.

Vancomycin, linezolid and rifampicin could be used effectively against methicillin-resistant staphylococci isolated from CRBSIs.

Background:The polymyxins including colistin are the "last resort" antibiotics for treatment of infections with carbapenem resistant Enterobacteriaceae. Recently, transferable colistin resistance mediated by the mcr-genes has been described in Enterobacteriaceae. The testing of colistin susceptibility is challenging. The aim of the work is to detect the occurence of mcr-1 and mcr-2 genes in phenotypically colistin-resistant carbapenem resistant Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) isolates using conventional polymerse chain reaction (PCR). Methods: One hundred and sixteen carbapenem-resistant E.coli and K.pneumoniae isolates were collected from patients admitted to different wards of Suez Canal University Hospitals (SCUHs) in Ismailia. Urine, endotracheal aspirates, blood, pus and sputum specimens were collected from different patients. Minimal inhibitory concentration (MIC) by broth microdiluton method was done to assess phenotypic colistin resistance. The colistin resistant E.coli and K.pneumoniae isolates were tested by conventional PCR to detect plasmid mediated mcr-1 and mcr-2 genes. Chi-square test was applied and p-value < 0.05 was considered statistically significant. Results: Forty three isolates out of 116 carbapenem resistant isolates (37.1%) were colistin resistant as shown by MIC by broth microdiluton method. The 43 colistin resistant E.coli and K.pneumoniae isolates were tested by conventional PCR to detect plasmid mediated mcr-1 and mcr-2 genes. Two mcr-2 genes and one mcr-1 gene were detected. Conclusion: Results revealed that the prevalence of colistin resistance among carbapenem-resistant E.coli and K.pneumoniae in SCUHs is concerning; further limiting potential therapeutic options. Plasmid mediated colistin resistance genes mcr-1 and mcr-2 is emerging in SCUHs which refer to a problem in the hospital as by horizontal transfer of this plasmid, the resistance can spread to many isolates in the hospital.

Introduction:The use of a mouthwash augments mechanical removal of plaque by brushing and flossing and helps maintain oral health through its antiplaque and antibacterial chemical properties. Aim: to evaluate and compare clinically and microbiologically the effectiveness of chlorhexidine and sodium hexametaphosphate mouth rinses on dental plaque in children. Materials and methods: This randomized clinical trial included 40 normal apparently healthy and cooperative children aged 8 to 10 years and selected from the outpatient dental clinic of Pediatric Dentistry Department, Faculty of Dentistry, Suez Canal University. Children were randomly divided into 2 groups as follows: Group I: included (20) children who rinsed with Chlorhexidine (0.2%) and Group II: included (20) children who rinsed with Sodium Hexametaphosphate (7%). Clinical evaluation was performed by measuring gingival and plaque indices scores at all evaluation periods. Microbiological evaluation was performed by direct examination of bacterial cultures. Results: Sodium hexametaphosphate 7% mouth rinse showed a superior effect compared to chlorhexidine 0.2% mouth rinse in terms of improvement in the clinical scores and decreasing the microbial counting. Conclusion: Sodium hexametaphosphate 7% mouth rinse is a promising candidate of mouthwashes.

Objectives This study aimed to evaluate the interleukin-6 (IL-6) levels and its relationship to stress, anxiety, and depressive symptoms among healthcare workers (HCWs) compared to controls during the COVID-19 pandemic. Methods A total of 80 HCWs in Suez Canal University Hospital in Ismailia, Egypt, and 80 controls were analyzed during the COVID-19 pandemic. The Depression, Anxiety and Stress Scale (DASS 21) questionnaire was used, and serum IL-6 level was determined in both groups. Results IL-6 levels were high in 81.2% (65) of HCWs compared to 36% (45) of controls ( P < .05). The DASS score was higher in participants with high IL-6 levels (>3 ng/mL) than in those with mild to moderate levels ( P < .05). The regression model revealed that the type of work as a healthcare staff, irregular or night shift, and stress were predictors of increased IL-6 levels among the studied sample ( P < .05) (odds ratio = 20.30, 2.44, and 2.04, respectively). Conclusion The IL-6 level and DASS score were higher in HCWs compared to those in controls during the COVID-19 pandemic. The type of work as a healthcare staff, stress, and irregular or night shift were predictors of increased IL-6 levels.