The present study carried out in Azady teaching general hospital in Kirkuk city during the period from August 2017 to May 2018, to determine the frequency of ToxA gene and ExoS gene in P. aeruginosa isolates. Pseudomonas aeruginosa (100) isolates had been obtained from (1260) different clinical specimens, High prevalence of P. aeruginosa found in isolates obtained from burn swabs. Regarding to genders and ages, P. aeruginosa presented in females (58%) more than males (42%), regarding to age groups, P. aeruginosa presented more frequently (26%) in age group (20-30) followed by children (1-10) years that formed (22%). Molecular detection of virulence genes had been done by using conventional PCR technique, regarding to prevalence of ToxA and ExoS genes, ToxA gene presented (96%), ExoS gene (92%). Regarding to the distribution of genes in clinical specimens, ToxA gene present (100%) in all clinical specimens except in urine samples (90.9%) and low percentage found in sputum (50%). ExoS gene present (100%) in all isolates, while ExoS gene less frequently presented in ear swab isolates ( 83.3%) than other specimens, followed by urine specimens (81.8%), in bronchial washes isolates ExoS gene was absent. This study aims to determine the frequency of ToxA gene and ExoS gene in P. aeruginosa isolates that obtained from different clinical specimens.