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Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions. K E Y W O R D S 3D cell culture, 3D-printing, bioreactor, optogenetics, recellularization 1036 | DANESHGAR Et Al. How to cite this article: Daneshgar A, Tang P, Remde C, et al. Teburu-Open source 3D printable bioreactor for tissue slices as dynamic three-dimensional cell culture models.