Plant‐derived exosome‐like nanoparticles (PDENs) are small vesicles released by multivesicular bodies mainly to communicate between cells and regulate immunity against pathogen attack. Current studies have reported that PDENs could modulate gene expression in a cross‐kingdom fashion. Therefore, PDENs could be a potential future functional food ingredient as their cross‐kingdom communication abilities were reported to exert multiple health benefits. Macrophage and other cells have been reported to absorb PDENs in a manner regulated by the membrane lipid and protein profile and the intactness of the PDENs lipid bilayer. PDENs could be extracted from plant materials by various techniques such as ultracentrifugation, immunoaffinity, size‐based isolation, and precipitation, though each method has its pros and cons. PDENs mainly contain lipid, protein, and genetic materials, mainly micro RNAs, which could exert multiple health benefits and functionalities when consumed in sufficient amounts. However, most studies on the health functionalities of PDENs were conducted through in‐vitro and in‐vivo studies, and its potency to be used as a functional ingredient remains a question as PDENs are sensitive to storage and processing condition and requires costly extraction method. This concise review features various exosome extraction methods, contents of PDENs and their roles, the health functionalities of PDENs, and its potency as a functional food ingredient.

Immunization is an effort to boost the immunity of someone actively against a disease, so when exposed to the disease will not get sick or only experience a mild ache. The main goal of immunization is to lower of pain, disability and death due to diseases that can be prevented by Immunization (PD3I). To achieve these objectives it must achieve coverage of the basic immunization of 91%. The achievement of basic immunization at the Gayam health center does not meet the target. One of the causes is not achieving the target because of the mother foctor in immunizated his son. This study was conducted to describe and analyze the relationship factors of mother in the achievement of basic immunization in region work of Gayam health center Sumenep regency. This study use cross sectional design. The subject were taken from a population by simple random sampling technique. The independent variables in this study were the age of mother, mother's level of education, occupation of mother, mother's level of knowledge, maternal attitudes and beliefs of mother. The results showed that the variables that associated in the achievement of basic immunization are mother's level of education (p = 0,020), mother's knowledge level (p = 0,000), beliefs of mother (p = 0.000) and the maternal attitudes (p = 0.000). While the variable age of mother and occupation of mother not associated in the achievement of basic immunization because the p value > 0.05. Conclusion of this study is factors of associated in the achievement of basic immunization are mother's level of education, mother's knowledge level, beliefs of mother and maternal attitudes. Therefore it is necessary for the addition of knowledge’s mother through the delivery of information, in addition health workers provides an explanation to the mother related to the incidence of post followup immunization so that mothers believe that immunizations have an impact well and the mother was able to behave better towards immunization.Keyword: basic immunization, beliefs of mother, maternal attitudes,     mother's knowledge level, mother's level of education

ObjectivesThis study aimed to determine colorectal cancer (CRC) risks among patients with gout through a follow-up study on a nationwide population-based cohort that included patients with gout and the general population in Taiwan.ParticipantFrom the Taiwan National Health Insurance Research Database, we identified 28 061 patients who were newly diagnosed with gout between 2000 and 2010 as the study cohort. We randomly selected 84 248 subjects matching in gender, age and baseline year as comparison cohort. The cohorts were followed up until CRC occurrence, withdrawal from the system of National Health Insurance, or Dec. 31, 2013.Primary and secondary outcome measuresCumulative incidences and incidence rate ratios (IRRs) of CRC between two cohorts were examined. The Cox proportional hazards model was used to evaluate risk factors associated with CRC development.ResultsDuring the 13-year follow-up, the incidence rate of CRC development in the gout cohort reached 2.44 per 1000 person-years, which was higher than the 2.13 per 1000 person-years in the control cohort (IRR=1.15; 95% CI 1.04 to 1.26). After adjusting for age, gender, urbanisation status and comorbidities, including hypertension, diabetes and hyperlipidaemia, gout showed no significant association with increased risk of CRC occurrence (adjusted HR=1.03; 95% CI 0.93 to 1.14).ConclusionsSimilar risks of CRC incidence were observed in patients with and without gout in Taiwan. Allopurinol and colchicine are commonly used as urate-lowering drug and anti-inflammation medication in Taiwan and had been shown to reduce the risk of CRC incidence. Thus, further pharmaco-epidemiological studies should be carried out to specifically assess the role of allopurinol in the relationship between gout and CRC.

Few effective therapies for cartilage repair have been found as cartilage has a low regenerative capacity. Extracellular vesicles (EVs), including exosomes, are produced by cells and contain bioactive components such as nucleic acids, proteins, lipids and other metabolites that have potential for treating cartilage injuries. Challenges like the difficulty in standardizing targeted therapy have prevented EVs from being used frequently as a treatment option. In this review we present current studies, mechanisms and delivery strategies of EVs. Additionally, we describe the challenges and future directions of EVs as therapeutic agents for cartilage repair.

Background: Stunting is a condition where the growth and development of a child fails during the first 1,000 days of life. The number of stunted children in Indonesia has increased from 35.60% in 2010 to 37.20% in 2013. Purpose: The aim of this study is to analyse the relationship between immunisation status and stunting in children 2–5 years. Methods: This is a secondary data analysis with cross-sectional study design. The data used for this study were obtained from Indonesia Family Live Survey wave 5. IFLS 5 was conducted in 13 provinces from October 2014 until April 2015. The data were collected from 1,048 respondents aged 2–5 years in the Indonesia Family Life Survey 5 with completed age and height data. The observed variables were age, sex, immunisation status, history of infection, mother’s height, mother’s age during pregnancy, living area, and region. Results: This research shows that there is a relationship between immunisation status (p = 0.01; OR =1.78; 95% CI = 1.26 < OR < 2.52), mother’s height (p = 0.00; OR = 1.41; 95% CI = 1.00 < OR < 1.98) and stunting in children aged 2–5 years. Conclusion: Immunisation status and mother’s height are associated with stunting in children aged 2–5 years.