Neomycin sulfate and hydrocortisone ear drops of veterinary pharmaceuticals used as an antibiotic and ant inflammatory to treat infections caused by bacterial infections in the middle ear of large and small field animals caused by gram positive and negative bacteria like, Staphylococcus aureus, Escherichia coli, Homophiles influenza, Klebsiella–Enterobacter species, Neisseria species, and Pseudomonas aeruginosa. The action of mechanism neomycin sulfate is inhibition of protein synthesis in bacteria cell which is lead to kill the bacteria and the action of hydrocortisone is ant inflammation in the infected area in animals. In order for this preparation to be researched and applied, several primary pharmaceutical preparations were prepared until the final and stable structure was reached. The amount of the active substance at room temperature was 100%. This activity is good and within the permissible limits According to the veterinary constitutions [90-110].This process involved several stages of collecting information in the materials involved in the formulation and from effective and added materials And then the preparation of the composition according to the international pharmaceutical specifications using the constitutions of the drug followed by the study of stability and resistance to the product temperature room and then send samples for field examination Department of Veterinary, was used in the treatment of cases of bacterial infections of the middle ear infections in cats use 3 or 4 drops in the affected ear three or four times for 3-5 days and results came very good according the clinical evaluation.

Sulphamethaxazole is antibiotic used to treat several types of bacterial infections and gives double effect when used with trimethoprim by 1 to 5 of sulphamethaxazole. It has a broad spectrum effect on the positive and negative bacteria of Gram and fungi. The objective of this research is to convert sulfamethasazole to nano sulfamethasazole by Sol-gel method by using additive material like acetic acid, distilled water and ethanol with temperature 80°C and probe sonicator machine as catalyst and hydrolysis factor. Nano sulphamethoxazole solution analyzed by atomic force microscope (AFM) was show average of nano particles at size (50±10) nm. Scanned by TEM and SEM shows nano particles as a band of hollow tubes with a diameter less than 100 nm., beside the EDS pattern show high peaks of sodium, carbon and oxygen along with some other constituents. The antibacterial activity of nano solution shows respectable inhibition activity compared to slandered solution.

Nano peel solution was prepared using Sol-gel technology at a temperature of (80) Celsius. A number of tests were performed to describe the properties and structure of Nano material, including scanning probe microscopy (SPM), which showed the symmetric cumulative distribution of the solution Nanoparticles and the average grain size equal to 64.5 nm and the identical distribution of the Nanoparticles with a diameter of 37.75 nanometers, with a measurement area ranging between (1531.23-1558.19) nanometers. Nano-solution analyzed with a scanning electron microscope (SEM), Inspect type (S50), with a magnification power up to X2000, where dense flakes of Nano particles with a diameter of (50 ± 10) nanometers were observed. The antibacterial activity of Nano-solution by using gram- positive bacteria St. aurous and gram-negative E. coli show the inhibition diameter of (St. aurous) was 27 mm and 25 mm in (E. coli).