Background: Denture base materials are subjected to sorption, a process of absorption and adsorption of liquids depending on the environmental conditions, also their fitting surface is subjected to microbial adhesion due to surface irregularities. Soft silicon liners are usually used with removable appliances to decrease load concentration on the hard and soft tissues. Silicon liners also undergo fluid sorption and microbial adhesion. Aim: This study was carried out to assess the effect of standardizing the oral hygiene conditions on the microbial load difference between conventional acrylic denture bases and silicon soft relining material (Mucopren soft) used to reline mandibular complete dentures in atrophied mandibles. Methods: Conventional complete dentures were constructed for 25 patients with flat atrophic mandibular ridges to be used for two weeks, salivary swabs were collected to evaluate oral flora attached on the fitting surface of the denture base and the buccal vestibule, soft liner was applied for all patients mandibular denture surfaces and the same approach was repeated after two weeks of the liner use. Results: Microbial load was found to be significantly higher in the fitting surfaces of the silicon soft liner, than in the conventional acrylic base fitting surfaces. Conclusion: Even under controlled oral conditions, Mucopren soft silicon liner incorporates greater microbial load than acrylic material thus, subjecting patients to numerous infections.

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Background: The efficiency of adhesives in enhancing the retention of complete denture has been well recognized.Yet the correlation of using adhesives with occlusal equilibration has not been investigated especially after introducing digitally fabricated complete dentures in order to overcome the drawbacks of the conventional processing technique of complete dentures.In addition, the competence of the prosthesis and its ability to improve the quality of life of the patient should be given great attention as improvement of patient`s life is the main goal of any medical intervention. Objective:The purpose of this crossover clinical study was to evaluate the influence of using adhesives with the digitally fabricated (3D printed) dentures on occlusal equilibrationandOral Health Quality of Life (OHRQoL) Materials and Methods: The Study was designed as crossover clinical trial, total of 27 subjects participated in the study, all the patients received digitally fabricated (3D Printed)set of complete dentures First: The patients were instructed to wear the complete denture for the first two weeks after that occlusal equilibration was evaluated in terms of occlusion time (OT) and dis-occlusion time (DT). Second: After two weeks the patients were instructed to use the same (3D printed) complete denture for another two weeks after application of denture adhesive then occlusal equilibration was evaluated again in terms of occlusion time (OT) and disocclusion time (DT). Oral Health-related Quality of Life (OHQoL) questionnairewas used to evaluate the patient satisfaction at the end of the follow up period of each denture set. Results:Regarding occlusion time,there was significant difference for all timings as mean ± standard deviation without adhesive and with adhesive were (0.56±0.018) and (0.31±0.024) respectivelyas P-value < 0.05.Regarding oral health effect on the quality of life, the studied domains revealed a significant improvement after adhesive application as the denture with adhesive showed lower scores than without adhesive. Conclusion: Within the limitations of this study and the given specific patient population, denture adhesives were found to significantly reduce occlusion time (OT) and disocclusion time (DT) durations in CAD/CAM complete dentures with fairly balanced occlusion, further improving denture stability and the patients Oral Health Quality of Life.

Background: Virulence factors of denture stomatitis had been contributed mostly to Staphylococcus aureus and Candida Albicans as an etiological basis in addition to immunodysfunction related to type of denture base and significant Ab-Ag reaction according to individual immune response. This study aimed to evaluate gold electro plating effect on Staphylococcus aureus and Candida Albicans colonization in addition to assessment of its cytotoxicity level. Subjects and Methods: Twelve maxillary completely edentulous patients were randomly divided into; Group I (control group): Completely edentulous patients rehabilitated with maxillary complete dentures reinforced with Cr-Co palatal plate and Group II (experimental group): Completely edentulous patients rehabilitated with maxillary complete dentures reinforced with Cr-Co palatal plate which were galvanic electro-plated by gold. Follow up assessment of Staphylococcus aureus, Candida Albicans and cytotoxicity level of Group I and Group II. The collected data were further statistically analyzed using SPSS and GraphPad statistical software. Results: Using One Way ANOVA followed by Tukey's post hoc test for multiple comparisons regarding microbiological evaluation, there was a significant difference among follow up periods for both groups. Using Independent t-test for comparison between both groups, was a significant difference especially after two weeks, 30 days and 45 days. While for cytotoxicity level, Gold-Plated experimental group showed insignificant higher cytotoxic effects than Cr-Co control group using Chi-Square test. Conclusion: Gold electroplating showed an antibacterial effect and lower rate of Candida Albicans colonization while it showed higher levels of cytotoxicity.

Background: Treatment challenges for completely edentulous patient have traditionally been described as a combination of esthetics, function and comfort. Some studies regarding complete denture esthetics were more important than function or comfort. Denture characterization is modification of the form of the denture base to produce a more natural appearance. Materials and Methods: Acrylic resin complete dentures had been divided into two groups according to flasking technique (group I as control group: conventional three pour flasking technique, group II as test group: silicone mask flasking technique). ΔE was calculated for both groups immediately and after seven days of exposure to different staining conditions (Smoke, Coffee and Tea). Results: Heat cured acrylic resin complete denture processed through silicone mask flasking technique showed statistically significant lower ΔE values (as P < 0.05) than conventionally processed heat cured acrylic resin complete denture. Conclusion: With the limitations of this study regarding color stability change (ΔE), depending on flasking technique; the conventional three pour flasking technique showed a higher amount of color change (lower color stability) than the silicone mask flasking technique regarding smoking, tea and coffee coloring agents.