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Methicillin-resistant Staphylococcus aureus (MRSA) infections are usually found in hospital settings and, frequently, in patients with open wounds. One of the most critical virulence factors affecting the severity and recurrence of infections is the biofilm; increasing antibiotic resistance due to biofilm formation has led to the search for alternative compounds to antibiotics. The present study aimed to use boric acid and potassium metaborate against MRSA infection in a fibroblast wound model. For this purpose, a two-part experiment was designed: First, MRSA strains were used for the test, and both boric acid and potassium metaborate were prepared in microdilution. In the second step, an MRSA wound model was prepared using a fibroblast culture, and treatments with boric acid and potassium metaborate were applied for 24 h. For the evaluation of the effects of treatment, cell viability assay (MTT assay), analysis of redox stress parameters, including total oxidant status and total antioxidant capacity analyses, lactate dehydrogenase analysis and immunohistochemical staining were performed. In addition, IL-1β and IL-10 gene expression levels were assayed. According to the results, potassium metaborate was more effective and exhibited a lower toxicity to fibroblast cells compared to boric acid; moreover, potassium metaborate decreased the level of prooxidant species and increased the antioxidant status more effectively than boric acid. The IL-1β level in the bacteria group was high; however, boric acid and potassium metaborate significantly decreased the expression levels of inflammatory markers, exhibiting the potential to improve the resolution of the lesion. On the whole, the findings of the present study suggest that boric acid and potassium metaborate may be effective on the tested microorganisms.

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Aloe vera is one of the medicinal plants whose importance has been better understood recently with its antibacterial, antimicrobial, antioxidant and anticancer properties. Although it is known that the aloe vera family has protective effects on neurons, the neuroprotective effect of the aloe barbadensis miller plant has not yet been fully explained. Glufosinate is structurally similar to glutamate and is an herbicide that blocks glutamine synthesis. Glutamate has been shown to induce cyclooxygenase-2 (COX-2), which causes toxicity. In the present study, neuron culture was exposed to Aloe vera barbadensis Miller plant extracts plant (25, 50, 100, 200, 400, 800, and 1600 µgr/ml doses) for 24 hours to protect against glufosinate (200 mM) and glutamate (10 -5 mM) toxicity. After 24 hours, MTT, TAC, and TOS analyzes were performed and the results were revealed. In our study, it was seen that the aqueous extract of aloe barbadensis miller plant, glufosinate, and glutamate, could preserve the vitality of neurons (89% protection in AVB 400 µgr/ml group). At the same time, it was seen that while increasing the antioxidant level in neurons, it decreased the oxidant level. The group that increases the antioxidant value best is AVB 400 µgr/ml (the group that increases it 1.4 times). When the findings were evaluated, it was concluded that aloe vera and its components may have a neuroprotective effect.