Eclipta alba is a medicinal herb belonging to the family of Asteraceae. Locally it is known as Kalokeshi and has a history of ethno-medicinal properties. The crude ethanolic extract of E. alba leaves was partitioned successively by solvents of different polarities. The crude extract along with different partitionates was evaluated for their possible antimicrobial, thrombolytic and membrane stabilizing activities. In this study, n-hexane soluble fraction (HXSF) showed 55.56% maximum activity against thrombosis in comparison with streptokinase (SK) 62.67%. For hypotonic solution- and heat-induced conditions, the n-hexane soluble fractionate (67.35 ± 0.50%) significantly inhibited the haemolysis of HRBCs by 67.35 ± 0.50% and 62.12 ± 0.69%, respectively as compared to the standard acetyl salicylic acid (77.91 ± 0.29%). The ethanol extract of the plant showed higher inhibitory action against Staphylococcus aureus, Bacillus subtilis, Proteus mirabilis and Pseudomonas fluroescens. The total flavonoid content was quite significant in ethanolic extract (142.17 ± 0.15 mg/g). Hence, the results of the present study revealed that the leaves of this traditional medicinal plant are a remedy for thrombosis, red blood cell membrane stabilization and microbial infections against some human pathogens.Dhaka Univ. J. Pharm. Sci. 14(2): 207-213, 2015 (December)

Background: The leaves of Sesbania grandiflora have been used in local traditional medicine since ancient times. In the present study we investigated, in vivo and in vitro, the potential health benefits of various fractions of the ethanolic extract of these leaves. Materials and methods: Crude ethanolic extract (CEE) of S. grandiflora leaves was partitioned into ethyl acetate soluble fraction (EASF), petroleum ether soluble fraction (PSF), carbon tetrachloride soluble fraction (CTSF), chloroform soluble fraction (CSF), and water soluble fraction (WSF). The extracts were evaluated for their thrombolytic, membrane stabilizing (anti-inflammatory), antimicrobial, and antidiarrheal activities. The results were compared to the effects of standard drugs: streptokinase for the thrombolytic, acetylsalicylic acid (ASA) for the membrane stabilizing, kanamycin for antimicrobial, and loperamide for the antidiarrheal activities. Results: For thrombolysis, EASF showed the highest % of clot lysis (59.6%) among

Present study aspires at the design of an immediate release formulation with prospective use of fexofenadine hydrochloride by exploring the effect of sodium starch glycolate as super disintegrant. Fexofenadine hydrochloride immediate release tablets (Formulations F-1, F-2, F-3, F-4 and F-5) using different ratios of sodium starch glycolate as a disintegrant were prepared by direct compression method. Standard physicochemical tests were performed for all the formulations. Dissolution studies of the formulations were done in phosphate buffer, pH 6.8 using USP apparatus II (paddle apparatus) at 50 rpm. Percent release of fexofenadine hydrochloride of formulations F-1, F-2, F-3, F-4 and F-5 were 89.98%, 90.98%, 92.95, 96.92% and 99.85%, respectively after 1 h and the release pattern followed the zero order kinetics. The release rate in the formulation F-5 was higher compared to other formulations and the studied market products. Sodium starch glycolate speed up the release of the drug from the core tablets, and the release of fexofenadine hydrochloride from tablets was directly proportional to the amount of sodium starch glycolate present in the formulations and there by produced immediate action.

The ethanolic plant extracts of Asparagus racemosus and their partitionates were assessed for thrombolytic, membrane stabilizing, antimicrobial and antioxidant activity in vitro. Methods: The collected plant was dried and grinded. The coarse powder was shocked in 2000 mL of 90% methanol for several days then filtrated. At 40°C the volume of crude ethanolic extract (CEE) was reduced by a vacuum rotary evaporator followed by concentrated aqueous ethanol extractive was fractionated into petroleum ether (PSF), carbon tetrachloride (CTSF), chloroform soluble fraction (CSF) and aqueous (AQSF) soluble fractions. Results: In thrombolytic assay among all the extractives, carbon tetrachloride soluble fraction (CTSF) showed the highest clot lysis (41.48 ± 0.43%) activity as compared with standard streptokinase (65.16 ± 0.16%). With respect to the membrane stabilizing activity, chloroform soluble fraction (CSF) profoundly inhibited the lysis of erythrocytes (80.86 ± 0.36%) induced by osmosis, whereas reference standard acetyl salicylic acid (ASA) resulted in (88.99 ± 0.39%) inhibition of lysis. On the other hand, CTSF showed slightly higher level of protection against heat-induced hemolysis (85.68 ± 0.75%) as same as with ASA, (85.68 ± 0.75%)). In antimicrobial investigation, crude ethanol extract (CEE) and their partitioning fractions exhibited moderate inhibition of growth of some examined bacteria. The total phenolic content was found maximum (930.94 mg of GAE/gm) in CEE of A. racemosus. The antioxidant potentials in term of DPPH free radical scavenging assay showed EC50 values 2.62 μg/mL by aqueous soluble fraction (AQSF) in comparison to ascorbic acid 2.48 μg/mL as a reference standard. Conclusions: This study was conducted to validate the A. racemosus plant as thrombolytic, membrane stabilizing agent, antimicrobial and antioxidant.