The main objective of this study was to determine the cytotoxicity of crude extract of the leaves of Lagerstroemia speciosa (Banaba). In vitro MCF-7 cell line and in vivo brine shrimp lethality bioassay methods were followed to investigate the cytotoxicity of the selected sample. In this study, the qualitative phytochemical assessment demonstrated the presence of alkaloids, carbohydrate, glycosides, saponins, terpenes, steroids, phenols and flavonoids in Banaba extract. Moreover, the present findings revealed the non-cytotoxic nature of L. speciosa leaves extract on both MTT-assay and brine shrimp lethality bioassay. Bangladesh Pharmaceutical Journal 22(1): 45-49, 2019

Eclipta alba is a medicinal herb belonging to the family of Asteraceae. Locally it is known as Kalokeshi and has a history of ethno-medicinal properties. The crude ethanolic extract of E. alba leaves was partitioned successively by solvents of different polarities. The crude extract along with different partitionates was evaluated for their possible antimicrobial, thrombolytic and membrane stabilizing activities. In this study, n-hexane soluble fraction (HXSF) showed 55.56% maximum activity against thrombosis in comparison with streptokinase (SK) 62.67%. For hypotonic solution- and heat-induced conditions, the n-hexane soluble fractionate (67.35 ± 0.50%) significantly inhibited the haemolysis of HRBCs by 67.35 ± 0.50% and 62.12 ± 0.69%, respectively as compared to the standard acetyl salicylic acid (77.91 ± 0.29%). The ethanol extract of the plant showed higher inhibitory action against Staphylococcus aureus, Bacillus subtilis, Proteus mirabilis and Pseudomonas fluroescens. The total flavonoid content was quite significant in ethanolic extract (142.17 ± 0.15 mg/g). Hence, the results of the present study revealed that the leaves of this traditional medicinal plant are a remedy for thrombosis, red blood cell membrane stabilization and microbial infections against some human pathogens.Dhaka Univ. J. Pharm. Sci. 14(2): 207-213, 2015 (December)

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Spondias pinnata is a medicinal herb belonging to the family of Anacardiaceae. Locally it is known as Amra and has a history of ethno-medicinal properties. The crude ethanolic extract along with different partitionates of the leaves of S. pinnata were evaluated for their possible thrombolytic and membrane stabilizing activities. The activities of the leaf extract were evaluated by using standard drugs; streptokinase for thrombolytic and acetyl salicylic acid for anti-inflammatory activities. In this study, among all partitionates the ethyl acetate soluble fraction showed highest % of clot lysis (58.06%) as compared to (69.23%) and (3.03%) exhibited by the standard streptokinase and water. On the other hand, ethyl acetate soluble fractionates also significantly inhibits the haemolysis of human erythrocyte membrane both in induced by hypotonic solution (65.33 ± 0.50%) and by heat (56.22 ± 0.69%), respectively as compared to (75.92 ± 29%) and (71.12 ± 0.26%) demonstrated by acetyl salicylic acid. Our study revealed that satisfactory amount of flavonoid content (132.27 ± 0.25) showed a significant and positive correlation between total flavonoid content with thrombolytic and membrane stabilizing activities.

The present study was carried out to investigate phytochemical, antioxidant; antimicrobial, thrombolytic activity and estimate total phenolic, total flavonoid content of Pandanus odoratissimus (p.odoratissimus) leaves of methanol extract. In thrombolytic activity, aqueous soluble fraction (AQSF) exhibited highest percentage (46.58 %) of potential to lyse blood clot compared to standard drug streptokinase (69.52 %). In antimicrobial assay, dichloromethane soluble fraction (DCMSF) explored the highest diameter of clear zone of inhibition against both gram positive (19.60 ± 0.12 mm) and gram negative (20.00 ± 0.20 mm) bacteria compared to standard antibiotic, Kanamycin (50.00 ± 0.19). Levels of antioxidant were determined by DPPH assay followed by calculated IC50 values of different Kupchan extracts. The methyl soluble fraction (MSF) showed the lowest level of IC50 value (36.70 ± 0.32 µg/mL) in comparison to ascorbic acid (12.48 ± 0.09 µg/mL) while MSF disclosed the maximum level (62.19 ±  0.26 mg of GAE/g of extract) of total phenolic content in the extracts of P. odoratissimus. This study was conducted to validate the P. odoratissimus leaves used as a folk medicine such as, antioxidant, thrombolytic, and antimicrobial potential.