Objectives were to evaluate the effect of prepartum energy intake on performance of dairy cows supplemented with or without ruminally protected choline (RPC; 0 or 17.3 g/d of choline chloride; 0 or 60 g/d of ReaShure, Balchem Corp., New Hampton, NY). At 47 ± 6 d before the expected calving date, 93 multiparous Holstein cows were assigned randomly to 1 of 4 dietary treatments in a 2 × 2 factorial arrangement. Cows were fed energy to excess [EXE; 1.63 Mcal of net energy for lactation/kg of dry matter (DM)] or to maintenance (MNE; 1.40 Mcal of net energy for lactation/kg of DM) in ad libitum amounts throughout the nonlactating period. The RPC was top-dressed for 17 ± 4.6 d prepartum through 21 d postpartum (PP). After calving, cows were fed the same methionine-balanced diet, apart from RPC supplementation, through 15 wk PP. Liver was biopsied at -14, 7, 14, and 21 d relative to parturition. Cows fed EXE or MNE diets, respectively, consumed 40 or 10% more Mcal/d than required at 15 d before parturition. Cows fed the MNE compared with the EXE diet prepartum consumed 1.2 kg/d more DM postpartum but did not produce more milk (41.6 vs. 43.1 kg/d). Thus, PP cows fed the EXE diet prepartum were in greater mean negative energy balance, tended to have greater mean concentrations of circulating insulin, fatty acids, and β-hydroxybutyrate, and had greater triacylglycerol in liver tissue (8.3 vs. 10.7% of DM) compared with cows fed the MNE diet prepartum. Cows fed RPC in transition tended to produce more milk (43.5 vs. 41.3 kg/d) and energy-corrected milk (44.2 vs. 42.0 kg/d) without increasing DM intake (23.8 vs. 23.2 kg/d) during the first 15 wk PP, and tended to produce more milk over the first 40 wk PP (37.1 vs. 35.0 kg/d). Energy balance of cows fed RPC was more negative at wk 2, 3, and 6 PP, but mean circulating concentrations of fatty acids and β-hydroxybutyrate did not differ from those of cows not fed RPC. Despite differences in energy balance at 2 and 3 wk PP, mean concentration of hepatic triacylglycerol did not differ between RPC treatments. Feeding RPC reduced the daily prevalence of subclinical hypocalcemia from 25.5 to 10.5%, as defined by concentrations of total Ca of <8.0 mg/dL in serum in the first 7 d PP. Pregnancy at first artificial insemination tended to be greater for cows fed RPC (41.3 vs. 23.6%), but the proportion of pregnant cows did not differ by 40 wk PP. Heifers born from singleton calvings from cows fed RPC tended to experience greater daily gain between birth and 50 wk of age than heifers from cows not supplemented with RPC. Feeding RPC for approximately 38 d during the transition period tended to increase yield of milk for 40 wk regardless of amount of energy consumed during the pregnant, nonlactating period.

The objectives were to determine the optimal feeding amount of choline in a ruminally protected form to reduce the triacylglycerol (TAG) concentration in liver and to increase TAG in blood plasma of dairy cows. Pregnant, nonlactating multiparous Holstein cows (n = 77) were blocked by body condition score (3.59 ± 0.33) and assigned to treatment at 64 ± 10 d before calculated calving date. Dietary treatments were top-dressing of 0, 30, 60, 90, or 120 g/d of ruminally protected choline (RPC; Balchem Corp., New Hampton, NY) ions to supply the equivalent of 0, 6.5, 12.9, 19.4, and 25.8 g/d of choline ions. Diets were formulated to exceed nutrient requirements for maintenance and pregnancy and fed in ad libitum amounts for the first 5 d. From d 6 to 15, cows were restricted to consume approximately 31% of their net energy requirements to simulate early lactating cows in negative energy balance. Methionine intake was maintained throughout each 15-d period. Liver was biopsied at 5 and 14 d and analyzed for TAG and glycogen. Blood was sampled on d 5 and 14 and plasma analyzed for glucose, insulin, cholesterol, β-hydroxybutyrate, long-chain fatty acids, and haptoglobin. On d 14, a mixture of saturated long-chain fatty acids, ground corn, and dried molasses (50:37:13) was offered (908 g, as-is basis) 10 h after the single daily feeding. Blood samples were collected for 19 h and plasma analyzed for TAG and cholesterol to assess apparent absorption of dietary fat. Mean dry matter intake and energy balance decreased from means of 9.5 to 3.3 kg/d and from 0.6 to -9.2 Mcal of net energy for lactation/d during the ad libitum and restricted feeding periods, respectively. Plasma concentrations of the lipid-soluble choline biomolecules, namely total phosphatidylcholines, total lysophosphatidylcholines, and sphingomyelin, increased with choline supplementation. Feed restriction increased plasma concentrations of β-hydroxybutyrate and free long-chain fatty acids, whereas those of glucose, insulin, and total cholesterol decreased. During feed restriction, concentration of hepatic TAG and plasma haptoglobin decreased linearly, whereas concentration of hepatic glycogen tended to increase quadratically with increasing intake of RPC. After fat supplementation, mean plasma concentration of TAG increased by an average of 21% with intake of RPC ions, peaking at intakes of ≥6.5 g/d of RPC ion. In summary, feeding RPC ions to cows in negative energy balance had increasing lipotropic effects on the liver when consumed up to 25.8 g/d, whereas feeding only 6.5 g/d increased concentrations of hepatic glycogen and TAG in the blood.

Measurements of body weight (BW), body condition score, wither height, calf birth weight, and calf sex were recorded by parity from 728 Holstein cows and heifers at the Purina Research Center during the 8-wk prepartum period. Data were compiled over 5 yr. Mean daily BW gain was 0.93 kg. Loss of BW at parturition was 11.1%. Mean body condition score was 3.35 (five-point scale where 1 = thin to 5 = obese) and did not change during the 8-wk period. However, body condition score was lower for second parity cows than for heifers or cows in third or greater parity. Wither height averaged 138 cm at first parity and increased 3 cm from first to second parity. During fifth and sixth parities, wither height peaked at an additional 2 to 3 cm. Birth weights for all calves averaged 41.4 kg. Male calves were 8.5% heavier than female calves. Calves born to cows in second or greater parity had increased BW by 7 to 8%. Mean BW of twin calves was 15% less than that of single calves.

A study was conducted to determine the impact of dietary CP concentration (13 vs. 20%) and feeding strategy (total mixed ration vs. separate feeding of the forage and concentrate) on reproductive performance of 57 early lactation dairy cows. Cows were assigned to one of four treatments in a 2 x 2 factorial arrangement. Rations composed of 40% forage (50% grass-legume silage:50% corn silage) and 60% concentrate (DM basis) were fed from d 5 to 100 postpartum. Cows fed a total mixed ration had lower ruminal ammonia and plasma urea N concentrations compared with cows fed separately. Feeding strategy groups showed no differences in reproductive performance. Cows fed a 20% CP ration had higher CP intake, higher ruminal ammonia, and higher urea N concentration in plasma and vaginal mucus. There were no differences between low and high CP groups in days to first observed estrus (24 vs. 27), days to first service (55 vs. 59), days open (72 vs 82) or services per conception (1.5 vs. 1.8). Days to first estimated ovulation were longer (22 vs. 17) in the high vs. low CP groups, and there was an interaction between protein intake and lactation number. Cows managed with an intensive program for detection of estrus and for reproductive health did not show differences in reproductive efficiency when fed 13 or 20% CP rations.

Forage Brassica spp. have been shown to produce adequate amounts of herbage during the cooler months of the fall, thus allowing extension of the grazing season in higher latitudes. The objective of this study was to determine if the nutritive quality of initial and regrowth herbage was influenced by planting and harvest date. In 1987, 1988, and 1989, three Brassica spp. (rape [B. oleracea L.], turnip [B. rapa L.] and turnip hybrid [B. rapa L. × B. pekinensis L.]) were planted in late May to early June, late June to early July, and late July to early August and were harvested each year at 64, 76, or 85 DAP. The plants regrew 60, 70, or 80 d and were harvested. Nutritive components measured were CP, NDF, ADF, Ca, Mg, and P. Nutritive levels declined with warmer temperatures and low soil moisture levels particularly during July and August. Neutral‐detergent fiber and ADF levels were higher, while the CP levels were lower in herbage from the earliest planting date compared with the later planting dates, regardless of species and year. The levels of Ca, Mg, and P were influenced by species and planting date. In general, the regrowth herbage had a lower fiber and a higher protein content than the initial herbage. The variation in nutritive quality among the three species was relatively small. Of more importance to the producer is that the quality of brassica herbage is more comparable to a concentrate than a traditional forage because of the relatively low fiber and high protein content.