No abstract

litut whole senumnsu, tulbtmnmuins, globulins, and fibrinogen were labeled with fluorescent dyes (sulfonhodamine B, Cl No. 45100 amid fluorescein isothiocyanate); albumin was also tagged with radioactive iodinse (I's') and tnitium (H3). In addition heterologous albumin was also Intheled witiu fluorescemut dyes tund radioiodine. The proteins were intravenously injected in

Sevemal Armusour and NIH pituitary hormone l)mePalations as well as other homologous serum l)roteimi fractions were labeled with fluorescent tlyes (sulphorhodamsuine B, CI. 45100, and fluoresceims isothiocyanate). Rats were injected intravenously with the different labeled proteins and the animssals killed at various intervals from 3 minutes to 10 days. Sections of the kidneys were studied under ultraviolet light (3,900 A). It was observed that all the labeled proteins passed the glomerular filter they behsave similarly in the kidney.

No abstract

Rat whole serum, albumin, globulins and fibrinogen fractions were labeled with fluorescent dyes (sulforhodamine B, CI No. 45100, and fluorescein isothiocyanate); albumin was also tagged with radioactive iodine (I131). Labeled serum fractions were intravenously injected in adult female albino rats and their decay in the circulation and histological distribution in the ovaries was studied. As a complement histoimmunological techniques using labeled fluorescent antibodies against rat serum fractions were also applied to the ovary sections of intact rats as well as of rats previously injected with unlabeled serum fractions. It was observed : 1) Labeled whole serum, albumin and globulins decay in the circulation with a half-life value of 2.8 days for the first, 2.6 days for the second and 3.1 days for the last. 2) Parallel with the fast component of this time decline curve, serum fractions diffuse extravascularly in the ovary and appear in the zona pellucida and cytoplasm of the oocyte in the growing follicles, via thecal capillaries, intercellular spaces of granulosa layer and antrum. 3) Histoimmunological techniques suggest that also endogenous circulating serum fractions may be transmitted to the follicles following the same route.