The study is carried out in the experimental field of Institute of Plant Genetic Resources "Konstantin Malkov"-Sadovo, Bulgaria during 2013-2015 growing seasons. Twenty two einkorn wheat accessions were evaluated to estimate of variability, heritability, genetic advance and associations among characters. The values of mean square for all twelve characters showed highly significant differences between einkorn genotypes. The phenotypic coefficients of variation were higher than genotypic coefficient of variation (GCV). High heritability accompanied with moderate expected genetic advances was recorded for number of spikelets per spike. High values for the genetic advance expressed as a percentage of the mean and moderate values for the heritability in broad sense were observed in the traits total number of tillers per plan and number of productive tillers per plant. The number of productive tillers per plant, grain weight per spike and spike length without awns had the strongest direct effect on grain yield per plant. The total number of tillers per plant had the highest negative direct effect on the grain yield per plant.Stepwise multiple linear regression analysis revealed that three traits included grain weight per spike, number of productive tillers per plant and total number of tillers per plant with R2 = 85.30%, had justified the best prediction model.

<p>The genetic diversity of gliadins in twenty two einkorn accessions preserved under long-term seed storage condition in the National gene bank of Bulgaria were evaluated, using acid polyacrylamide-gel electrophoresis (Acid-PAGE). In total, 64 polymorphic bands and 22 gliadin patterns were identified. Thirty four different mobility bands and 21 gliadin patterns were identified in the ω-gliadin zone, 12 bands and 16 patterns were noted in the γ-gliadins, 17 patterns and 12 mobility bands were found for β-gliadins and six bands with five different α -gliadin patterns were determined. The genetic diversity index (H) was the highest for ω-gliadins (0.950), followed by β-gliadins (0.924) and γ- (0.914), respectively and the lowest value was detected in α-gliadin patterns (0.120). Cluster analysis based on the UPGMA method and Nei and Li similarity coefficients classified all the genotypes into 3 main groups. No relationships between genetic diversity, geographic origin and the genotypes were observed. The results of cluster analysis justify the high level of genetic variation among investigated einkorn accessions.</p>

The aim of the current study is to trace the changes in the length of the growing season in accordance with the accumulated effective temperatures, rains and plant nutrition during the vegetation period, as well as the influence of these indices on the grain yield. The study was conducted in the period 2015 -2016 in the experimental field of Institute of Plant Genetic Resources -Sadovo with the local variety B3E0025 from the National Genebank in Sadovo. The experiment was made in block method in four repetitions. Three sowing times were made -autumn, winter and early spring and two options of N fertilizationwithout additional N and 3 kg/da input of active N substance in tillеring phase. The beginning of the basic phenophases has been observed. As a result of the study it was established that the vegetation period in T. monococcum L. has 114-238 days' duration and accumulation of effective temperature of 2266.0-2714.71°C. The length of the growing season and the necessary accumulated temperature depends on the sowing period, the predecessor, the amount of nutrient substances and soil moisture. The extension of the critical phases of autumn sowing favours the obtaining of higher yield. The significance of the effective temperatures decreases with additional N input.

Abstract. The objective of this study was to investigate the reaction processes of seedlings originating from wheat seeds after they were subjected to accelerated ageing, which imitated low-temperature storage. Germination, vigor, morphological characteristics, relative chlorophyll content and antioxidant enzyme activity were measured in seedlings and young plants of wheat (Triticum aestivum L.) after processes of accelerated ageing of the seeds. The ageing procedures of the seeds were done according to the standard conditions (40±0.5°C and 100% air relative humidity) of the International Seed Testing Association for 72 and 120 hours of treatments of the Bulgarian varieties Geya-1 and Sadovo 772. For the control were used seeds, which were not subjected to the accelerated ageing conditions. The coleoptile of 5 days seedlings and second leaf of 10 and 15 days plants in seedling stage, following the seed accelerated ageing procedure, was measured in the laboratory and the greenhouse. The seed germination rate and vigor, fresh and dry weight of growing coleoptiles were inhibited after different periods of ageing exposure and well correlated with increased accumulation of total hydrogen peroxide, malondialdehyde content, guaiacol peroxidase activity and rate of cells membrane stability index. The negative changes of chlorophyll content index, fresh and dry weight and leaf area of the leaves of young plants descending from aged seeds sowed in pots were found. It was established that the modern variety Geya-1 was more tolerant to the applied ageing condition than the older Sadovo 772. In conclusion, we considered that accelerated ageing could be used as a model for estimation of seed deterioration in wheat after long-term storage.

The aim of this study was to assess the degree of variation between 32 accessions of wild einkorn (Triticum boeoticum Boiss.) on the basic morphophysiological and anatomical characteristics of the flag and subflag leaves. The experiment was carried out during 2016 -2017 growing seasons in the randomized block design in four replications and 10 m 2 plot size. Significant differences among the accessions for all studied characters were recorded. The epidermis of the studied 32 accessions was constructed by strongly elongated prosenhyme cells with flexous walls. The stomatas were with oval to elliptic shape, about 1.5 times longer than wide. The most variable character was the total chlorophyll content. Accessions with numbers B6E0416, B6E0413, B6E0398 and B6E0392 had the largest amount of chlorophyll pigments exceeding the average standard almost twice. The water-to-biomass ratio in the flag leaf was the greatest for B6E0378, B6E0389 and B6E0401, while for the subflag l eaves B6E0379, B6E0401 and B6E0385 were with the highest amount of water per unit of dry mass. The correlation between intensity of transpiration and the fresh and dry mass of leaves were slightly negative for flag leaf and slightly positive for subflag leaf. The water content of the subflag leaf had a stronger influence on the morphophysiological parameters compared to the water content of the flag leaf. PC-analysis grouped accessions according to similarity on the basis of investigated morphophysiological and physiological characters in two components in the factor plane. Keywords-wild einkorn, anatomy of leaf, morphophysiological characters of leaves, correlation, PC-analysis.