Gwadar Port is the mega project of ongoing developmental projects in Balochistan which is shaping the economy of the World. The port is creating opportunities and possibilities for promoting regional and international shipping and it will resuscitate trade links between China and CARs being the closest route to warm waters. Gwadar Port has vast region to influence; stretching up to several breakaway states of the former Soviet Union in the north, to Iran, the Gulf, the Middle East and East Africa in the west, to India and Sri Lanka in the south. Moreover, this deep port is serving the Gulf and East African ports with fast feeder services. It has deep-water sea complementary to Karachi and Bin Qasim ports for enhancing cargo shipments and therefore it will be a mother port for Asia in the coming years.

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Magnolia biondii Pamp is an important ornamental tree species widely grown and used as a rootstock in the propagation of different Magnolia varieties. In the current studies, anatomical, physiological and endogenous hormones were studied to check the effect of IBA 750 mg/L on the adventitious rooting and to provide theoretical and technical support for the propagation of Magnolia biondii Pamp through stem cuttings. Two thousand stem cuttings were prepared and divided into two groups i.e., IBA treated cuttings and water control. For the evaluation of antioxidant enzyme activities, and endogenous hormones levels, samples were collected on the day of planting and each 5th day and further steps were carried out in the laboratory according to the protocols and proper precautions. For the anatomical observations, samples were collected on the 13th, 15th, and 17th day for IBA treated cuttings while 21st, 23rd, and 25th day for control. Collected samples were preserved in the FAA solution and further observations were carried out in the laboratory. Anatomical observations showed that it took 13 days for the differentiation of root primordia to the appearance of young adventitious roots in IBA treated cuttings, while it took 21 days to develop primordia in the control. Antioxidant enzyme activities involved in ROS were significantly higher in the IBA treated cuttings compared to control. POD showed a peak on the 13th day before the emergence of roots in IBA treated cuttings while it showed a peak on the 21st day in the control. PPO showed a peak on the 21st day in the IBA treated cuttings while it showed a peak on the 29th day in the control. SOD showed a peak on the 17th day in IBA treated cuttings, while it showed a peak on the 25th day in the control. Exogenous application of IBA enhanced the endogenous IAA and GA3 levels compared to CK, while it reduced the levels of ABA continuously at the time of rooting and then increased gradually. Inclusively, our study suggests that IBA 750 mg/L is efficient for the rooting of Magnolia biondii Pamp cuttings, as it enhanced the process of antioxidant enzyme activities, endogenous hormones levels and reduced the time of root formation which is evident from the anatomical observations.

Opak River watershed is one of the river watershed in special region of Yogyakarta Province which has important role as water reserve for the community. The eruption of Merapi volcano that often occurs has damaged riparian ecosystem of the rivers in Opak river watershed. To restore Ecosystems in Opak river watershed Conservation during 2016-2018. Tree planting have been conducted five times in riparian ecosystem and Mangrove ecosystem during 2016-2018, At least 17000 individuals have been planted in tree area comprising ( 5000 individual of 25 species in upstream, 2000 individuals of six species and 10000 individuals of Rhizophora spp. Biodiversity survey have been conducted two time in July-August 2017 and April 2018 at three area in Opak river watershed (up stream, middle stream and down stream). The results shows a total of 291 species of plants, 84 birds species, 18 species of herpetofauna, 10 species of mammals, 35 species of fish, 206 species of insects, 26 species of macrozoobentos (mollusca), and 113 species of plankton in three study areas has been identified in Opak Watershed during 2017-2018. The presence of species in each study area as well as the composition of the number of individuals per species is shows difference between the survey in 2017 and 2018 .This is also showed in the number of those diversity indexes. This Biodiversity monitoring is planned to be continued least 5 times which will be conducted in every years.