In rice cultivation, farmers tend to use chemical fertilizers and pesticides. The prolonged intensive use of agrochemicals can decrease soil quality. Chemical pesticides can be replaced with biopesticides to maintain and improve soil fertility, also to prevent the attack of plant-disturbing organisms on crops. The aim of this study was to determine the total population abundance of soil bacteria and soil quality after the application of biopesticides in the swampland in Jejangkit District. This research was accomplished in Jejangkit District, Barito Kuala Regency, South Kalimantan, which started from June to December 2019. The study used a Randomized Completely Block Design (RCBD) with three treatments and six replications. The treatments are Super Intensive and Actual Swamp (RAISA), Indonesian Agricultural Environment Research Institute’s (IAERI) Biopesticide, Galam Biopesticide. The variety of rice used is Inpara 2. The plot size is 900 m2 by applying the Jarwo Planting system. The results revealed that the application of biopesticides could increase the total population of soil bacteria from 104 to 106 cfu mL−1. Moreover, N and P nutrient content increase from low to moderate (0.19 to 0.4) % and (13.25 to 38.87) mg P2O5 100g−1, respectively.

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