Anganwadi worker (AWW) is a multipurpose worker employed under the Integrated Child Development Scheme, India. They are the community's primary link with health services and an important agent for behavioral change for improving QoL. The AWW undergo training at the beginning of their career and once in every 3 years, and this training includes various issues regarding the improvement of physical, psychological, social, environmental, and other aspects of life. Therefore, they can assess their QoL in a rational manner. Hence, we undertook this study to assess the QoL of AWWs of Mandya city, Karnataka, India. Materials and Methods A cross-sectional study was conducted between September and December 2011 on AWWs. We included all the workers from all the 96 Anganwadi centers of Mandya city, India, to ensure full coverage, after obtaining the permission from the concerned authority. All the workers who gave consent were included for the study. To assess the QOL, we used the World Health Organization Quality of Life-short version (WHOQoL-BREF) that includes four domains (physical health, psychological, social relations, and environment). The WHOQoL-BREF questionnaire is available in 19 languages including Kannada, the local language. The Kannada version of WHOQoL-BREF has been validated Background: Quality of life refers to a subjective evaluation, which is embedded in a physical, psychological, social, and environmental context. Objectives: To determine the quality of life of anganwadi workers (AWWs) of Mandya city, Karnataka, India. Materials and Methods: A cross-sectional study was conducted from September to December 2011. All the AWWs from Mandya city were included. The World Health Organization Quality of Life-short version (WHOQoL-BREF) in Kannada, the local language, was administered to assess their quality of life. Results: The mean age of AWWs was 40.9 ± 8.3 years. Majority of them had completed their high school, and most of them felt their overall quality of life was good. The mean scores were 69, 63, 58, and 56 for social, physical, psychological, and environmental domains, respectively. Conclusion: Of the four domains of quality of life, the social and physical domains were slightly higher than the environment and psychological domains.