Leaf temperatures in a Koch fully climatized gas-exchange chamber as designed by Siemens and in a similarly equipped open-air reference were measured with horizontally and vertically inserted thermocouples on Nerium oleander L. On a sunny day with only little air movement and an average air temperature of 20-4 °C, leaf over-temperatures in the gas-exchange chamber were lower on average by 2-2 K. The extent of reduction of over-temperature in the chamber is determined by the reduced global radiation in the chamber and the differences of wind velocities in chamber and reference. Differences in the ventilation intensity in the chamber have no demonstrable influence on the leaf over-temperatures. The over-temperatures of the reference leaves, on the other hand, depend to a large degree on air velocity. The changed radiation and air flow conditions in the chamber as compared with open-air conditions have consequences for the physiological reactions of the enclosed plant and must be taken into account when comparing results from gas-exchange measurements with open-air conditions. For further improvements of gas-exchange measurement equipment, air flow conditions and radiation quantity and quality might be starting points INTRODUCTION