Background: The incidence of infections caused by yeast, particularly species of Candida and facultative anaerobic bacteria like Staphylococcus aureus and Lactobacillus acidophilus has increased dramatically during the past decade. Topical and systemic antifungal agents and anti microbial agents may be indicated to control oral candidiasis and periodontal and endodontic disease. The development of resistance is an emerging trend that may threaten the clinical eff ectiveness. Aim: An in vitro study to evaluate the antimicrobial effi cacy of ginger extract on Candida albicans, S. aureus, L. acidophilus, using fi ve diff erent polar organic solvents. Materials and Methods:The crude solvent extracts of ginger were prepared and subjected to antimicrobial assay using the Agar disk diff usion method to determine the zone of inhibition. The solvent extract with a signifi cant zone of inhibition is subjected to gas chromatography and mass spectrometry, to identify the bioactive constituent in the ginger extract. Result: Hexane extract of ginger shows high antimicrobial property, and the bioactive component was found to be zingiberene. Conclusion:The solvent extracts of ginger were proven to have eff ective antimicrobial property.