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In this present research, the Gold nanoparticles (GA-AuNPs) produced from Gelidiella acerosa, marine algae using green synthesis method was evaluated for its antidiabetic, antibacterial and antioxidant activity. Well-developed GA-AuNPs were effectively characterized using advanced analytical instruments like HRTEM, UV-visible, SEM and XRD. This analysis exposed GA-AuNPs to be present with spherical, hexagonal structure and crystalline nature. The average size of GA-AuNPs particles was estimated to lie between 5.81 nm to 117.59 nm by using Debye-Scherer equation. The enzyme inhibitory property of (IC 50 ) of G. acerosa aqueous extract and synthesized gold nanoparticles from G. acerosa against α amylase and α-glucosidase enzyme was found to be 2.1 ± 0.01, 3.7 ± 0.01 µg/mL and 2.8 ± 0.02, 4.1 ± 0.01, respectively. Further antioxidant assessment of GA-AuNPs were examined using DPPH and FRAP assays, the results exhibited that the strong antioxidant activity were observed in all the concentrations when compared with standard. Finally antibacterial activity was evaluated the GA-AuNPs against gram positive and gram negative bacteria, the strong antibacterial activity was observed in both the bacterial strains when compared with standard antibiotic. The GA-AuNPs from marine algae was exhibited to be strong inhibitory activity against alpha amylase and alpha glucosidase enzyme, antioxidant activity and antibacterial activity, these GA-AuNPs are projected to have potential biological applications.

Enhalus acoroides (Linnaeus f.) Royle belongs to the Family: Hydrocharitaceae is an abundantly growing seagrass in coastal areas of Gulf of Mannar. Sea grasses contain very good potent therapeutic properties for challenging diseases. The current investigation is to green synthesis Ag-NPs from Enhalus acoroides and characterize silver nanoparticles (Ag-NPs) and evaluated a-glucosidase inhibition activity. Furthermore, the characterization of the same using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDX), and followed by Transmission Electron Microscopy (TEM). Surface Plasmon resonance exhibited the development of Ag-NPs in UV-Visible spectra at 419 nm. The FTIR examination was done to find and read the moiety accountable aimed at the bioconversion of silver ions. The crystalline form was observed in the XRD examination. The synthesized Ag-NPs were polydispersed spherical AgNPs as confirmed by EDAX and stabilized in the solution with the spherical shapes further confirmed by TEM analysis designate in the reading of 2-100 nm. The findings of the study shown that Ag-NPs of promisingly proved have strong a-glucosidase inhibitory activity by which AgNPs with an IC 50 47 lg/ml. This current research report is first to account on the green synthesis of silver nanoparticles from seagrass E. acoroides, because there is no literature survey and investigations on Enhalus acoroides. Hence, we suggest that Ag-NPs synthesized from E. acoroides might be a significant resource of a-glucosidase inhibitors preparation that may benefit diabetes treatment.

Objective: The present investigations are to mycosynthesis and characterization of gold nanoparticles conjugated with doxorubicin and evaluated anticancer activity. Methods:The characterization of the gold nanoparticles using ultraviolet-visible spectroscopy. FTIR investigations were carried out to find and read the functional group responsible designed at the bioconversion of gold ions and crystalline arrangement of gold nanoparticles was detected in the XRD study. The gold nanoparticles conjugated with doxorubicin were treated against MCF-7-dox resisted breast cancer cells and observed the in vitro cytotoxicity by MTT assay, SCGE (Comet), Apoptosis and Mito-potential assay. Further more we determinate the mRNA expression of ABCB1 gene and cDNA was synthesized from the mRNA for amplification of the ABCB1 gene corresponding to the specific primer.Results: Surface Plasmon resonance showed the development of gold nanoparticles in UV-Visible spectra at 537 nm. The synthesized gold nanoparticles were polydisperse spherical and it was determined by EDAX and stabilized in the solution to the spherical shapes further confirmed by High-resolution transmission electron microscope analysis designate in the reading of 2-100 nm. The anticancer assays were given significant results and the mRNA expression of ABCB1 gene and cDNA was amplified as directly proportional to the expression of ABCB1 gene. Conclusion:We propose that gold nanoparticles synthesized and conjugated with doxorubicin from G. lucidum might be a significant resource of drug delivery for anti-cancer preparation that may advantage breast cancer treatment.

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