[EFFECTS OF PALM OIL SLUDGE SUBTITUTION FOR SYNTHETIC N FERTILIZER ON GROWTH AND YIELD OF SWEET CORN]. Palm oil sludge (POS) contains high concentrations of organic nitrogen dan its can serve as an alternative for synthetic nitrogen fertilizer. Objective of this study was to compare the growth and yield of sweet corn as amended with different compositions of synthetic N fertilizer and palm oil sludge. Six compositions, each containing 138 kg N/ha were assigned as the treatment and arranged in a randomized complete block design with three replications. These were 100% synthetic fertilizer, 80% synthetic fertilizer + 20% POS, 60% synthetic fertilizer + 40% POS, 40% synthetic fertilizer + 60% POS, 20% synthetic fertilizer + 80% POS, and 100% POS. The results of this study indicated that the plot amended with 60% synthetic fertilizer + 40% POS produced the highest plant stature (176.4 cm), stem diameter (2.40 cm), leaf area (8712.82 cm2 ), plant fresh weight (375.19 g), plant dry weight (136.68 g), and husked ear length (17.93 cm). However, the highest observed husked ear yield was only about 61.6 % the yield potential of the sweet corn variety.
This study aimed to investigate research about cytotoxicity correlation level of Phaleria macrocarpa (Scheff) Boerl also known as Mahkota dewa leaves methanol extract against MCF-7 cell in vitroto investigate the relationship between them in the goal to investigate alternative low cost herbal medicine agents to fight breast cancer. Cytotoxicity properties of samples against MCF-7 breast cancer cell lines was performed by using the Microtetrazolium (MTT) assay against MCF-7 cell line. The correlation between concentration of crude and cytotoxic activity was interpreted by statistical analyses. The study showed that P. macrocarpa leaves extracts showed cytoxicity activity against breast cancer MCF-7 cell lines which IC50 concentration showed at 15 µg/mL Correlation between concentration of extract and cytotoxicity property (absorbance value) were founded in weak relationship (R = 0.372, R2 = 0.138). It could be effect of many different compounds in the P. macrocarpa leaves methanol extracts may cause the pharmacological interactions, so lower or higher concentration will be antagonistic effect on absorbance or cell viability. Further study on its mechanism pathway on revealing against breast cancer could be explored. Furthermore, the natural product derived from P. macrocarpa leaves methanol extracts have potential use as alternative drugs against breast cancer.
[CHANGE IN SOIL CHARATERISTICS AND PERFORMANCE OF UPLAND RICE VARIETIES IN COASTAL AREA AS AMENDED WITH BIOFERTILIZER AND BIOCOMPOST]. Dryland in the coastal area has good potential for the cultivation of upland rice to reach food self-sufficiency and the development of future agriculture. Low fertility of the land the area can be overcome by using appropriate technology such as the use of superior varieties, bio-fertilizers, and bio compost. This study aimeds to (1) determine chemical and biological properties of coastal land to improve the growth of upland rice (2) find out the growth of upland rice in the coastal area using low input technology and (3) obtain upland rice varieties with high adaptability to a coastal area environment. The experimental design used was a split-plot design with the main plot of 3 upland rice varieties, namely Inpago 10, Serantan, and Local Variety, while the subplots are were fertilizer inputs namely [double inoculant P solubilized microbial p (pf) + K solubilized microbially + N fixation microbial N], [double inoculant P solubilized microbial (fma) + K solubilized microbially + N fixation micarobia], [biocompost at a dose of 10 tons/ha], and [inorganic fertilizer recommended by BPTP ie 200 kg Urea/ha, 100 kg SP36/ha, 100 kg KCl/ha]. The results showed that the coastal area has the potential for the development of upland rice cultivation. Also, the double inoculants of biological fertilizers were able to increase plant nutrient uptake, soil biological characteristics, and the growth of upland rice as compared to controls. Specifically, the best treatment is produced by application of [double inoculant microbial solvent p (pf) + microbial solvent K + microbial N fixation] combined with upland rice Inpago variety 10.
Potato microtubers are the miniature of conventional seed tubers grown in the field. Producing potato microtuber in vitro at high temperature by applying many kinds of tuber promoting substances is similar to producing potato in the field at low elevation by applying the same tuber promoting substances. The objective of this research was to evaluate the performance of seven tuber promoters in inducing potato microtubers at high temperatures. Micro cuttings (6 weeks old) were grown at the liquid media of MS for stolon induction. Six weeks afterward, the media were moved to microtuber inducing media supplemented with tuber promoters ((FD, FD+10 COU, FD+20 COU, FD+30 COU, FD+40 COU, FD+50 COU, and IPB). The media were incubated in the dark at 20 + 2 o C and 29 + 2 o C. The results showed that, in general, high temperature inhibited crop growth and tuber formation. Individually, tuber promoter formula significantly affected microtuber emergence, in which IPB showed the slowest treatment in producing microtubers, the smallest number of tuber produced, and the smallest size of tubers. The interaction between temperature and tuber promoters were significantly affected the tuber formation, in which the fastest tuber formation (7,0 days ) was found in FD + 40 COU and the highest percentage of plant producing tubers was found in FD + 30 COU. Moreover, the highest number of microtuber and the heaviest weight of microtuber were found in FD (no Coumarin). It was safely concluded that all tuber promoters were best performed at low temperature. Should anyone wish to make those tuber promoters work better at high temperature, it is recommended to increase the concentration of Coumarin added to the formula of tuber promoting substances.Keywords: high temperature, in vitro, potato microtubers, tuber promoter INTISARI Umbi mikro kentang merupakan miniatur umbi kentang yang digunakan sebagai benih untuk penanaman di lahan. Produksi umbi mikro kentang pada suhu tinggi dengan zat pemacu pembentukan umbi (tuber promoter) dijadikan sebagai simulasi produksi kentang di dataran rendah dengan menggunakan formula tuber promoter. Tujuan penelitian ini adalah mengkaji pembentukan umbi mikro kentang pada suhu tinggi dengan berbagai formula tuber promoter. Stek mikro kentang berusia 6 minggu tanpa akar dan pucuk ditanam pada media cair untuk menginduksi pembentukan stolon. Pada usia 6 minggu stek mikro dipindahkan ke media induksi umbi mikro yang telah diberi
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