The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) is one of the most severe fruit tree pests in China, causing huge economic losses to fruit production. So far, there are few detailed reports on the rearing protocols of G. molesta. In this study, we compared the longevity, 10-d fecundity, flight ability, and the activity of major energy metabolism enzymes in the flight muscles of G. molesta under three feeding regimes (supplement with sterile water, supplement with 10% honey solution and starvation) of the adult moths. The results showed that the longevity, 10-d fecundity, and flight parameters (cumulative flight distance and time, maximum flight distance and duration, and the average flight speed) of adult moths when supplemented with sterile water or honey solution were significantly higher than those of moths that were starved. There were no significant differences in the 10-d fecundity, flight parameters, and the activity of major energy metabolism enzymes of flight muscles between moths that were supplemented with sterile water or 10% honey solution. The flight muscles of G. molesta mainly used carbohydrates as an energy source when sterile water and honey solution were supplemented, and the moth mainly used lipids as an energy source under starvation. Considering the cost and potential for diet contamination during mass-rearing, supplying sterile water is considered a cost effective option for food substitution of adult G. molesta.

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Grapholita molesta is one of the most serious pests in fruits orchards. Flight performance of male insects and fecundity of female insects are important quality control parameters when moths are mass-reared for use in environment-friendly control strategies such as the sterile insect technique (SIT). However, information about flight performance, fecundity, and ovary development of G. molesta at different ages is scarce. In this study, we used a flight mill information system to measure the flight ability of female and male adults of G. molesta at different ages, and evaluated fecundity and ovarian development of female adults at different ages. The results demonstrated that the flight parameters (cumulative flight distance, cumulative flight time, maximum flight distance and maximum flight duration) of female and male G. molesta varied with age. Six-day-old female moths and three-day-old male moths were the strongest fliers, whereas the fecundity of one-day and two-day-old female moths was significantly lower than that of three to seven-day-old females. Five-day-old females had the highest fecundity. Their ovaries demonstrated mature eggs in the lateral and middle oviducts as of the third day post-emergence. It is suggested that the optimal age for moths to be released in SIT programs is three days, and moths older three days can be used for mass-rearing in a factory.