The effect of traffic on the content of lead and cadmium in grass morphological parts—leaves, shoots, and inflorescences—was studied. The samples were taken on a part of the European route E30 (Siedlce by road). The following plants were tested: Dactylis glomerata, Arrhenatherum elatius, and Alopecurus pratensis. During the flowering of grasses, the plant material was collected at distances of 1, 5, 10, and 15 m from the edge of the road, on the strip of road with a length of 9 km. In the collected plant parts, the content of lead and cadmium using the atomic absorption spectroscopy (AAS) method was determined. The effect of distance from the road on the content of lead and cadmium was evaluated using regression equations. Average lead content in the above parts of tested grass species was 3.56, while cadmium 0.307 mg kg−1 dry matter (DM). Lead content in plants of Alopecurus pratensis (average 4.11 mg kg−1 DM) was significantly higher than in other grasses. The lowest cadmium content, significantly different from the other species, was found in plants of Arrhenatherum elatius (0.251 mg kg−1 DM). Distance of sampling sites from the roadway significantly affects the differences in the content of cadmium and lead in plants. Analyzed aboveground plant organs of studied grasses were significantly different in contents of lead and cadmium. There were species differences in the proportions of cadmium concentration in various organs of plants. The obtained results indicate the possibility of species composition selection of grassland sward in areas with a higher risk of heavy metals associated with dust sedimentation.
Replicated three times, the research was conducted in the experimental field between 2011 and 2014. Three soil conditioners with the following trade names: UGmax, EkoUżyźniacz, and Humus Active Papka were used in the experiment, separately or together with NPK fertilisers. They were all used on plots sown with two species of grass, Dactylis glomerata of the Bora variety and Lolium perenne of the Info variety. The plant material from both grass species was tested for the concentration of ADL fraction (% DM), cellulose (% DM), and hemicellulose (% DM). It was found that the concentration of cellulose, hemicelluloses, and the ADL fraction was significantly higher in the biomass of Dactylis glomerata than in the biomass of Lolium perenne. The grass from the plot with the UGmax soil conditioner applied had the highest amount of cellulose and hemicellulose. The lowest amount of those organic compounds was found in the grass treated with UGmax together with mineral fertilisers and in plants treated with Humus Active, together with mineral fertilisers. However, the fertilisers and conditioners did not increase the ADL content in both grass species.
In 2011 a study was carried out analyzing the effects of road traffic on bioaccumulation of zinc and copper in selected species of dicotyledonous plants growing on adjacent grasslands. To do the research the plants were sampled from the 9-km-long Siedlce bypass, a part of the international route E-30. They were collected during the flowering stage, at following distances from the road: 1, 5, 10, 15 m. The content of zinc and copper was determined with the AAS method, with dry mineralisation done before. The highest concentration of the elements, regardless of the distance from the road, was found in Taraxacum spec. Among the tested plants, the lowest zinc content was in Vicia cracca, and the lowest copper content in Rumex acetosa. The limit for copper content was exceeded in Taraxacum spec. and, slightly, in Achillea millefolium growing at the roadside, closest to the roadway.
A study on the cultivation of Festulolium braunii cv. 'Felopa' was carried out using polyurethane rings with a diameter of 36 cm and a height of 40 cm, which were sunk into the ground to a depth of 30 cm and filled with soil material. In this experiment, Kelpak SL was used as a bioregulator. It consists of natural plant hormones such as auxins (11 mg in dm3) and cytokinins (0.03 mg in dm3). The experimental factors were as follows: A1-control; A2 – 20% solution of the growth regulator; A3 – 40% solution; and A4 – 60% solution. The preparation was applied to all three regrowths in the form of spray, at a rate of 3 cm3 ring-1, at the stem elongation stage. The full period of this experiment was in the years 2010–2011. During this time, detailed investigations were carried out on aboveground biomass yield (g DM ring-1), number of shoots (pcs ring-1), leaf blade length (cm), width of the leaf blade base (mm), leaf greenness index (SPAD). The study showed a significant effect of the growth regulator on the formation of Festulolium braunii biomass. However, its highest effectiveness was observed when the 60% solution was applied
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