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Neonicotinoids are a popular and widely-used class of insecticides whose heavy usage rates and purported negative impacts on bees and other beneficial insects has led to questions about their mobility and accumulation in the environment. Neonicotinoid compounds are currently registered for over 140 different crop uses in the United States, with commercial growers continuing to rely heavily on neonicotinoid insecticides for the control of key insect pests through a combination of in-ground and foliar applications. In 2008, the Wisconsin Department of Agriculture, Trade and Consumer Protection (DATCP) began testing for neonicotinoids in groundwater test wells in the state, reporting detections of one or more neonicotinoids in dozens of shallow groundwater test wells. In 2011, similar detection levels were confirmed in several high-capacity overhead center-pivot irrigation systems in central Wisconsin. The current study was initiated to investigate the spatial extent and magnitude of neonicotinoid contamination in groundwater in and around areas of irrigated commercial agriculture in central Wisconsin. From 2013–2015 a total of 317 samples were collected from 91 unique high-capacity irrigation wells and tested for the presence of thiamethoxam (TMX), a neonicotinoid, using enzyme-linked immunosorbent assays. 67% of all samples were positive for TMX at a concentration above the analytical limit of quantification (0.05 μg/L) and 78% of all wells tested positive at least once. Mean detection was 0.28 μg/L, with a maximum detection of 1.67 μg/L. Five wells had at least one detection exceeding 1.00 μg/L. Furthermore, an analysis of the spatial structure of these well detects suggests that contamination profiles vary across the landscape, with differences in mean detection levels observed from landscape (25 km), to farm (5 km), to individual well (500 m) scales. We also provide an update of DATCP’s neonicotinoid monitoring in Wisconsin’s shallow groundwater test wells and private potable wells for the years 2011–2017.

The Colorado potato beetle (Leptinotarsa decemlineata) is a major agricultural pest of solanaceous crops. An effective management strategy employed by agricultural producers to control this pest species is the use of systemic insecticides. Recent emphasis has been placed on the use of neonicotinoid insecticides. Despite efforts to curb resistance development through integrated pest management approaches, resistance to neonicotinoids in L.decemlineata populations continues to increase. One contributing factor may be alterations in insect fatty acids, which have multiple metabolic functions and are associated with the synthesis of xenobiotic-metabolizing enzymes to mitigate the effects of insecticide exposure. In this study, we analyzed the fatty acid composition of L. decemlineata populations collected from an organic production field and from a commercially managed field to determine if fatty acid composition varied between the two populations. We demonstrate that a population of L. decemlineata that has a history of systemic neonicotinoid exposure (commercially managed) has a different lipid composition and differential expression of known --

BACKGROUND The purse-string suture (PSS) is a simple, reversible method for reconstructing skin cancer defects. Its utility may be underappreciated in head and neck reconstructive surgery. OBJECTIVE To evaluate the utility of the PSS as an adjunct to the reconstructive ladder when reconstructing head and neck skin cancer defects. MATERIALS AND METHODS This prospective nonrandomized cohort included 109 consecutive adult patients with head and neck defects resulting from skin cancer resection. The PSS was placed in every patient after resection. Patients were evaluated before and after suture placement, and final reconstructive methods were recorded. RESULTS The PSS resulted in a mean defect area reduction of 77.1% (p < .001) and was used in the final reconstruction in 79 (72.5%) patients. The PSS reduced the number of patients requiring adjacent tissue transfer (11), regional tissue transfer (3), and free tissue transfer (1) by 69.4%, 70%, and 80%, respectively. Fifty patients underwent reconstruction in the operating room. After intraoperative placement of the PSS, it was determined that 22 (44.0%) of these defects could have been reconstructed in the clinic (p = .003). CONCLUSION The PSS dramatically reduces defect size, simplifies reconstructive techniques, and often permits reconstruction to be performed in the clinic rather than the operating room.

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