Summary: In the world practice of treating burns, acellular matrices have been used for quite a long time. However, the budget for treating one burn patient in Kazakhstan does not exceed $1000. This amount does not cover the costs for procurement of foreign-made xenograft dressings. Because the cattle breeding sector is very well-developed in the country, a domestic xenograft is produced by decellularization and sterilization of the cattle peritoneum, which costs only $10. This case report outlines how we used this matrix in a patient with partial thickness burns. A 45-year-old woman was admitted to the burn department with second stage burns on her back and right shoulder. The burn area comprised 10%, according to the Lund Browder chart. Once formal consent was obtained from the patient, an occlusive dressing was applied from the decellularized cattle peritoneum. Good adhesion of the dressing to the wound bed was noted. The patient was discharged from the hospital on the tenth day. It took 23 days to reach complete epithelialization. No adverse effects were noted. We believe that further studies conducted by our research team will allow this innovative, low-cost, easy-to-apply biologic dressing to be widely used in the therapeutic treatment of burns.

This research aimed to assess the effect of bone allograft combined with platelet-rich plasma (PRP), recombinant human bone morphogenetic protein-2 (rhBMP-2), and zoledronic acid (Zol) on bone formation. A total of 96 rabbits were used, and femoral bone defects (5 mm) were created. The rabbits were divided into four groups: (1) bone allograft with PRP (AG + PRP), (2) bone allograft with rhBMP-2 5 μg (AG + BMP-2), (3) bone allograft with Zol 5 μg (AG + Zol), and (4) bone allograft (AG). A histopathological examination was performed to evaluate bone defect healing after 14, 30, and 60 days. The new bone formation and neovascularization inside the bone allograft was significantly greater in the AG + PRP group compared to AG and AG + Zol groups after 14 and 30 days (p < 0.001). The use of bone allograft with rhBMP-2 induced higher bone formation compared to AG and AG + Zol groups on days 14 and 30 (p < 0.001), but excessive osteoclast activity was observed on day 60. The local co-administration of Zol with a heat-treated allograft inhibits allograft resorption as well as new bone formation at all periods. In conclusion, this study demonstrated that PRP and rhBMP-2, combined with a Marburg bone allograft, can significantly promote bone formation in the early stage of bone defect healing.

Background and Objectives: Homogeneous and xenogenic bioengineering structures are actively used as wound coatings in treatment of burns and have already shown their effectiveness. Nevertheless, the disadvantage of such dressings is their high cost. This issue is particularly challenging for developing countries in which the incidence of burns is the highest one. With such needs taken into account, the research team developed and clinically tested a new wound coating based on decellularized bovine peritoneum (DBP). Materials and Methods: A multicenter randomized clinical trial was conducted to evaluate DBP. The following variables were considered in the research study: the number of inpatient days, the number of dressing changes, the level of pain experienced during dressing changes, and the condition of wounds at the time of the follow-up examination. Results: The research involved 68 participants. It was found that the patients who were treated with a DBP experienced less pain with less changes of dressings. However, the number of inpatient days and wound healing failed to demonstrate statistically significant difference compared to the control group. Conclusions: In the given research, DBP showed efficacy in improving patients’ quality of life by reducing pain and the number of dressings’ changes. However, when comparing this research study with the studies of other animal-derived wound coverings, there were a number of differences and limitations in the parameters. Thus, the results requires further study for a greater comparability of data. Given the above, we expect that DBP will become an inexpensive and effective treatment for burns in developing countries.

Introduction: Chronic osteomyelitis occurs in 3-25% of cases after open fractures and in 1-8% of cases after surgical treatment of closed fractures. It accounts for 3 to 10% in the structure of purulent surgical diseases. Traditional treatment methods many times do not provide a complete cure for osteomyelitis. In modern medicine, many experimental models have been created on different types of laboratory animals in order to reproduce the model of chronic osteomyelitis and subsequently improve its therapy. Of all the experimental animals, we chose rabbits for the application of the developed method for the treatment of chronic osteomyelitis and for the selection of the optimal model of bone tissue suppuration.The objective was to conduct a clinical, radiological and histological analysis of various models of chronic osteomyelitis in experimental animals. Materials and methods:The experiment was carried out on 90 outbred rabbits. All rabbits were randomly divided into 5 groups. The rabbits were kept in a room with controlled temperature (16-21 °C) and relative humidity (45-65%). The rabbits were placed in special cages -2-3 rabbits per each. Surgical intervention was performed under general anesthesia. Surgical access was made along the anterior surface of the distal metaepiphyseal area of the left femur. All animals were intraosseus injected with Staphylococcus aureus as an infectious agent. The development of chronic osteomyelitis was assessed by clinical, radiological, microbiological, histological and statistical methods.Results: The study showed that X-ray and histological methods play an important role in the analysis of experimental models. The signs of osteomyelitis development can be seen on the 14th day after the operation with the help of the X-ray method, while wound healing, weight and temperature cannot be indicators. According to the results of the experiment, the signs of chronic osteomyelitis developed in all 5 groups, but its activity was different. Histological examination showed that osteomyelitis developed differently in different groups, fibrosis developed unevenly.