Among the first of comparatively few studies made on the effect of alcohol on unicellular organisms were those of Calkins and Lieb (1902) and Woodruff (1908). These workers used Paramecium and in general concluded that ethyl alcohol, in "moderate" concentration, acts as a stimulus in sustaining the vitality of these cells.On the contrary, Matheny (1910) and Estabrook (1910) found no evidence that alcohol acts as a periodic or continued stimulus. In minute doses, 2% or less, it apparently has no effect on Paramecium while in doses of 3% or greater, it kills them. Daniel (1909) working with another genus, Stentor, found that ethyl alcohol causes an increase in growth rate. He also states that comparatively weak solutions of alcohol (1% or less) are ineffective, while a 4% solution causes