than in the control group. In the formalin test, the licking time for THME at doses of 300 and 500 mg/kg was significantly shorter ( p < 0.001) compared to the control group in the first phase of the formalin test, whereas in the second phase only the dose of 500 mg/kg showed an antinociceptive effect. In addition, THME at doses of 300 and 500 mg/kg significantly increased the latency time in the tail flick test ( p < 0.05 and p < 0.001, respectively) and in the hot plate test ( p < 0.01 and p < 0.001, respectively) compared to the control group. Conclusions: These results show that THME had antinociceptive activity using several models of nociception, and they suggest that the effect is mediated by the participation of both peripheral and central antinociceptive mechanisms. AbstractObjective: The aim of this study was to evaluate the antinociceptive activity of the methanol extract of Tabebuia hypoleuca stems (THME). Materials and Methods: The animals were divided into 5 groups of 8 mice for each test (negative controls, positive controls, and 3 groups treated with THME at doses of 150, 300, and 500 mg/kg, p.o.). The antinociceptive effect of THME was evaluated using the writhing, formalin, tail flick, and hot plate models in mice. Results: In the writhing test, THME (150, 300, and 500 mg/kg) produced significantly ( p < 0.001) fewer writhes induced by acetic acid Significance of the Study• In this study Tabebuia hypoleuca was shown to have antinociceptive effects mediated by the participation of both peripheral and central antinociceptive mechanisms. The T. hypoleuca species could become a new therapeutic option for the treatment of pain.

Rhizophora mangle L. is a vegetal species with high disponibility in Cuba for guarantying economical factibility by drug development. Preview studies showed promissory pharmacological results by the use of this plant in the treatment of gastroduodenal ulcers. The present work involved another evaluation of the active extract important by the development of a new drug. It was evaluated by intestinal absorption in vitro and in situ models and anti-diarrheic activity in rats. The polyphenolic fractions were separated from the aqueous extract and its cytoprotective activity was studied on gastric ulceration induced by ethanol plus hydrochloric acid in rats. Some toxicological studies were carried out. We made a validation of HPLC method by the analysis of one mark in the tablet development. The aqueous extract showed high absorption, and high anti-diarrheic effect. The low-molecular weight polyphenolic fraction showed the highest level of gastric protection. No toxicological signs were obtained by it. HPLC method evidenced linearity, precision, sensitivity and accuracy.