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In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Leaf extract has been assayed against Alternaria alternata which is responsible for leaf spot disease of chilli. Cold and hot extraction of leaf extract was prepared in different organic solvents, which were subsequently recycled by rotary vacuum evaporator. Antifungal activity of prepared plant extracts were determined by poison food technique. In cold extraction highestpercent extractive value was obtained with 50% alcohol extract and in hot extraction highest percent extractive value was obtained with Methanol extract. In antifungal activity 100% alcohol crude extract and partial purified acetone extract was show Maximum inhibition of Alternaria alternata. Mancozeb and water were used as standards. Results suggest that Lawsonia inermis. Leaf extract can be used to develop a bio control agent against Alternaria alternata. Lawsonia inermis plant can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

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Causal Organism COVID-19 is a disease caused by the novel coronavirus (SARS-CoV-2). Positive-sense ssRNA viruses are about 30 kb long. Coronaviruses have a broad spectrum of action and affect multiple organisms. Origin of Disease The disease outbreak has been initiated in Wuhan, China, in December 2019. The disease has been originated from the wet animal market of Wuhan City, where a large number of peoples have come in contact with animals. It is known to infect the neurological, respiratory, enteric, and hepatic systems. SARSCoV-2 is highly infectious, and its outbreak is worldwide (national and international level) and becomes pandemic. Control Presently, the number of cases continues to rise at a global level, and it is clear that these viruses pose a significant threat to public health. Consequently, extensive treatments, vaccines, and drugs have been developed by researchers to control the transmission of infection. This led to the isolation of patients that were administered a variety of treatments. Special attention and guidelines have been given by various government organizations to protect or reduce transmission between children, healthcare providers, and old-aged peoples.

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