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Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different types of secondary metabolites synthesized by plants which are play important role in biotechnological application and also in antimicrobial activity. In the present study were carried out to determine in vitro the antifungal activity of alcoholic, hydroalcoholic and aqueous crude extracts of Polyalthia longifolia leaf against Rhizoctonia solani fungus. All these extracts i.e., 100% aqueous, 100% alcohol and 50% hydro alcohol used as 10mg/ml concentration with acetone solvent to control of Rhizoctonia solani. The results revealed that the extract that most effectively inhibited growth was found to be 100% alcoholic leaf extract. Finding in this study confirmed that plant extracts can be used as to develop the plant extract-based bio-formulation to control plant pathogens to reduce the dependence on the synthetic fungicides which are ecological safe and cheap.