Some species of the genus Brachyspira such as Brachyspira pilosicoli, Brachyspira intermedia and Brachyspira alvinipulli are pathogenic species capable of producing disease in laying hens. In our country, the presence of B. pilosicoli and other species of Brachyspira has been reported in pigs and dogs but there is no record of their presence in poultry. Fecal and cecal content samples from 34 laying hens of 4, 12 and 24 months of age from two farms were analyzed by isolation, biochemical tests and PCR. B. pilosicoli and Brachyspira spp. were identified in samples taken from laying hens of 12 and 24 months of age.
RESUMODeterminou-se, por meio de um sistema de monitoramento de suínos em fase de crescimento e em matadouro, a presença de patologias como indicadoras de problemas na população de referência. O estudo foi realizado em uma criação intensiva de ciclo completo, durante nove semanas. Foram determinadas as prevalências dos diagnósticos clínico-patológicos obtidos durante um período de nove semanas. Utilizaram-se cinco grupos de 43 leitões, com pesos de até 19kg, considerados de baixa performance. Estimaram-se, por intervalos de confiança de 95%, as prevalências acumuladas em cada monitoramento. Obteve-se correlação de 0,99 (P<0,0001) entre as prevalências anuais com as nove semanas de estudo, mostrando correspondência com o achado em matadouro. Amostras em semanas alternadas de três grupos de 43 animais são bons indicadores das patologias que afetam os suínos em crescimento, mesmo aquelas de baixa freqüência ou muito letais.Palavras chave: suíno, matadouro, prevalência, diagnóstico clínico-patológico 43, 86, 129, P<0.0001) Ulvesaeter, 1979;Morrison et. al., 1985). A
ABSTRACT
SUMMARYThe aim of this study was to determine the colonization and serologic state of piglets and sows of different ordinal number of farrowings. Between 78 and 81 sows, multiple-vaccinated against Mycoplasma hyopneumoniae (Mh), were sampled (from 1 st to 9 th parity) at day 0 of gestation, 8 weeks of pre-farrowing and post-farrowing, and 163 piglets at two weeks old, taking blood samples (for enzime-linked immunosorbent assay test, ELISA) and nasal swabs (for polymerase chain reaction, PCR). In addition to the percentages of seropositive and PCR positive, other values were obtained to determine the concentration of antibodies and to analyze the dynamics of antibodies in sows and piglets. The percentage of seropositive sows and title values were both high. The same was observed in piglets, allowing to conclude that the multiple vaccinations of the first ones guarantees a high level of antibodies in their offspring, but not 100%. The sows of intermediate parity number showed a higher variability in those values that reflected concentration of antibodies; at day zero of gestation the sows of first farrowing showed these lower values. At post-farrowing, younger sows and their offspring showed the highest percentage of nPCR positive. Sows (and piglets) showed a higher concentration of antibodies when increasing the number of ordinal farrows, although this can be due to vaccination and the repeated exposure of the animals to Mh under field conditions.Palabras clave: Mycoplasma hyopneumoniae, hembras, lechones, colonización, ELISA, nPCR.
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