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The decrease in antioxidants A, C and E in HIV positive children is due to increased utilization of antioxidant micronutrients because of increased oxidative stress caused due to free radicals.

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Background: The objective is to study the oxidative stress in obese and non-obese children by assessing the biomarkers of lipid peroxidation and antioxidant status, Malondialdehyde (MDA) and Ascorbic acid (vitamin C) respectively. Childhood obesity is a growing global epidemic that requires attention due to the burden placed on the healthcare system for children and adults. Consumption of fatty foods and a high sugar, fat diet, and no exercise qualify as the main reasons for obesity among children and adults. Childhood obesity is connected with an increased risk of various diseases such as diabetes, cardiovascular, stroke, certain types of cancer later in life, social problems and depression among youths. Obesity is also characterized by chronic low grade inflammation with permanently increased oxidative stress (OS). Over-expression of oxidative stress damages cellular structures together with under-production of anti-oxidant mechanisms, leading to the development of obesity-related complications.Methods: The study involved 25 obese children for Vitamin C, 20 obese children for Malondialdehyde (MDA) and 18 non obese children for both Vitamin C and MDA in the age group of 5-14 years, without any complications. This study was conducted at L.T.M.M College. Plasma Ascorbic Acid was estimated colorimetrically by using 2,6- dicholrophenol indophenol dye and similarly Malondialdehyde was estimated colorimetrically by MDA-TBA colored complex.Results: The study showed significantly higher values of MDA and lower level of Vitamin C in obese children as compared with non-obese children.Conclusions: The levels of lipid peroxidation marker Malondialdehyde (MDA) is higher and level of antioxidant marker Ascorbic Acid (Vitamin C) is lower in obese children as compared with non-obese children. Thereby increasing oxidative stress and hence the oxidative damage to cells.

Background:The objective is to assess the effect of medical nutrition therapy on biochemical parameters like Vitamin C and Malondialdehyde by estimating oxidative stress in HIV positive malnourished childrens' sample. Any alteration in the antioxidant markers can help to assess the effectiveness of MNT as a therapeutic agent. There is limited data on the use of Ready to Use Therapeutic Food (RUTF) in India. Therefore, the study was conducted to determine the effect of prepared Ready to Use Therapeutic Food (RUTF) also termed as Medical Nutrition Therapy (MNT) in HIV positive malnourished children. Methods: In this prospective comparative study, pre MNT and post MNT sample as well as the parametersMalondialdehyde and Vitamin C were analyzed in 19 HIV positive malnourished children in the age group of 6-12 years at L.T.M.M. College. HIV positive malnourished children showed adverse effects on nutrition as well as immune system. Thus, to supply adequate micronutrients and macronutrients, the subjects were treated with Medical Nutrition Therapy (MNT). Results: The post MNT HIV positive malnourished childrens' samples revealed significant results than pre MNT HIV positive malnourished childrens' samples. Conclusions: The level of antioxidant marker (vitamin C) increased and peroxidation marker (MDA) decreased post medical nutrition therapy. The increased post levels of vitamin C activity helped in the eradication of excess free radicals, thereby reducing oxidative stress and hence the oxidative damage to cells as well as inhibiting lipid peroxidation (which is indicated by decreased post level MDA).