Gynodioecy is a sexual system in which females and hermaphrodites co-occur. In most gynodioecious angiosperms, sex is determined by an interaction between mitochondrial malesterility genes (CMS) that arise via recombination and nuclear restorer alleles that evolve to suppress them. In theory, gynodioecy occurs when multiple CMS types are maintained at equilibrium frequencies by balancing selection. However, some gynodioecious populations contain very high frequencies of females. High female frequencies are not expected under balancing selection, but could be explained by the repeated introduction of novel CMS types.To test for balancing selection and/or the repeated introduction of novel CMS, we characterised cytoplasmic haplotypes from 61 populations of Lobelia siphilitica that vary widely in female frequency.We confirmed that mitotype diversity and female frequency were positively correlated across populations, consistent with balancing selection. However, while low-female populations hosted mostly common mitotypes, high-female populations and female plants hosted mostly rare, recombinant mitotypes likely to carry novel CMS types.Our results suggest that balancing selection maintains established CMS types across this species, but extreme female frequencies result from frequent invasion by novel CMS types. We conclude that balancing selection alone cannot account for extreme population sex-ratio variation within a gynodioecious species.

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In the pictures taken by the camera trap, the photo of a leopard hunting a jungle cat is rare. This type of evidence makes it easier to understand the current status of wildlife and also implement further conservation actions. This study was conducted from November 2020 to December 2020 in the mid-hill regions of Nepal. We installed the camera traps in l*1 km grid cell covering about 60 km2. Out of 36 camera trap locations, 1 leopard preying on a jungle cat was captured in one location. Our study concluded that carnivores could capture another sympatric family because of the lack of natural prey species. Therefore, conservation of the natural prey species including small carnivores or mammals is also essential to conserve the leopard population.