There is evidence to suggest that the development of a stable microvasculature at the site of a critical-sized bone defect or fracture aids in repairing or regenerating bone. Identifying a tissue engineering scaffold that optimizes bone tissue and blood vessel development could improve regenerative capabilities. In this paper we study the proliferation and directed differentiation potentials of endothelial colony forming cells and mesenchymal stem cells cultured on electrospun polycaprolactone matrices and compare them with data obtained for composite polycaprolactone-hydroxyapatite, polycaprolactone-hydroxyapatite/b-tricalcium phosphate and polycaprolactone-small intestine submucosa electrospun matrices. Polycaprolactone-hydroxyapatite and polycaprolactone-hydroxyapatite/b-tricalcium phosphate fibers on average displayed a twofold increase in fiber diameter and average pore-size area as compared with polycaprolactone or polycaprolactone-small intestine submucosa scaffolds. X-ray diffraction showed that significant additions of hydroxyapatite, hydroxyapatite/b-tricalcium phosphate and small intestine submucosa were present in the composite scaffolds. Incorporating hydroxyapatite or hydroxyapatite/b-tricalcium phosphate into the polycaprolactone fiber increased the modulus and ultimate tensile strength significantly. Both endothelial colony forming cell and mesenchymal stem cell proliferation was twofold greater on polycaprolactone-small intestine submucosa scaffolds; whereas on polycaprolactonehydroxyapatite and polycaprolactone-hydroxyapatite/b-tricalcium phosphate scaffolds only endothelial colony forming cell proliferation was observed to be significant. Alkaline phosphatase analysis for mesenchymal stem cell-seeded scaffolds indicated that only polycaprolactone-small intestine submucosa scaffolds displayed significant increases after 10 days of culture, suggesting an osteoblast phenotype. Electrospun polycaprolactone-small intestine submucosa scaffolds stimulated proliferation of both cell types and directed mesenchymal stem cell differentiation, providing a stable platform to investigate the potential of endothelial colony forming cell in directing bone tissue repair or regeneration.

The microstructure and the chemistry distribution of AlNiCo 9 samples were characterized by the X-ray diffraction, magnetic force microscope, field emission scanning electron microscopy and transmission electron microscope. An interface of a high Al content was formed near the FeCo-rich phases with a size of about 30 nm. S elements mainly combined with Ti to form titanium sulfide bars with the length between 70-150 μm, while S elements was not confirmed in the nanostructured FeCo-rich phase and AlNi-rich phase. Si and Nb preferably existed in the NiAl-rich phase, and a higher content Nb near the Cu precipitate boundary was observed. Moreover, the magnetic domain structure of AlNiCo 9 was also studied.

1J50 alloy is one of the major iron–nickel compositions,well-known for high flux density, low coercivity and high saturation induction.In this study,the microstructure and magnetic domain struture of the soft magentic alloy 1J50 before and after heat treatment (HT) were studied using scanning electron microscopy (SEM)、transmission electronmicroscopy (TEM) and magnetic force microscope (MFM). The results showed that grain size and domain size were small before HT,there was high-density dialocation in the alloy.After HT,grain size increased, the internal stress, dislocation and other defects were reduced,magnetic domains became wider,the number of the domain decreased and the exchange energy between the magnetic domain reduced,leading to the decrease of the coercivity and increase of permeability.It was also found that the curie temperature was not changed after heat treatment. The research can provide theoretically a reliable way for preparing Fe-Ni soft magnetic alloys with high performance.

With the development of the oil industry, oilfield sewage, as a big pollutant, treatment has become an important issue. Since the complex components of oily sewage, untreated oily sewage may lead to serious environment problem if discharged at will.So it must be treated before discharged or returned into the layer.In this study, some treatment methods is introduced, and their treatment effects are illustrated.