of nutrients for DM, OM, CP, CF, NDF, ADF, cellulose and hemicellulose were significantly (P<0.05) higher in treatment groups (T 1 and T 2) than the control (T 0), except those for EE and NFE. The serum enzymes aspartate amino-transferase (AST), alanine amino-transferase (ALT) and alkaline phosphatase (ALP) did not differ within groups. Yeast supplementation did not adversely influence red blood cells, PCV and white blood cells, except a significant (P<0.05) improvement in haemoglobin concentration. The results indicated that inclusion of live yeast culture in 50:50 concentrates: wheat straw based TMR resulted in improved rumen fermentation and digestibility of nutrients, without affecting biochemical profile in Kankrej cows.