Seed priming is a useful technique, applied prior to planting, which partially hydrates the seeds to a point of germination process initiation, followed by drying, which prevents radicle emergence. The objective of this study was to evaluate the effect of various seed priming treatments on germination parameters of maize at different temperatures. Seeds were primed in water (hydropriming) and KNO3 solution (0.1% and 0.5%) by soaking at 25°C for 17h. Germination parameters were tested at 25°C, 15-25°C and 15°C. Seed priming had a positive effect on some seed germination parameters at low and mixed temperature. Treatment with 0.5% KNO3 had the most improving effect. This treatment reduced MGT, T50 and increased GE at low and mixed temperature. Priming showed no positive effect on the final germination. This study showed that seed priming with KNO3 could be used to achieve better seedling establishment at low temperature conditions in maize.
One of the main advantages of camelina (Camelina sativa (L.) Crantz) is its wide environmental adaptability and extreme drought tolerance. The availability of both winter and spring camelina biotypes, characterized by different seed sizes, raises the question about possible differences in their response to drought stress at the emergence stage. To address this, a germination test was set up in controlled conditions, comparing six winter and six spring genotypes with differing seed sizes (ranging from 1.83 to 0.88 g/1000-seeds) under increasing levels of osmotic stress (0, −0.4, −0.8, −1.2, −1.4, −1.6 MPa) using polyethylene glycol (PEG). Camelina withstands mild level of osmotic stress (−0.4 MPa) without significant decrease in germination. Even at −1.2 MPa after 10 d, it still had 75% germination. Significant differences in germination were observed between biotypes, where spring biotypes performed better than winter ones. Shoot and radicle lengths were significantly diminished by imposed osmotic stress, but shoot growth seemed more impacted. In general, spring biotypes had longer shoots and radicles than winter ones. Seed size played a role in the response of camelina to drought, but it depended on biotype and stress level imposed. In particular large seeded spring types had the highest germination percentage and resulted less impaired by osmotic stress, otherwise among the tested winter types the small seeded ones were the best performing. The presented data could be useful for breeding purposes for selecting the appropriate camelina type for sowing in drought-prone regions.
The aim of this study was to investigate field emergence parameters, number of plants that reached reproductive stage and yield of seed lots with different count of abnormal seedlings. In standard germination test these seedlings are discarded from the count of germinated seed, presuming that they will not develop into normal plants. Five lots of hybrid NS 640 with different percentage of abnormal seedlings were tested. Results showed that in favourable environment conditions, large number of abnormal seedlings emerged, reached reproductive stage and participated in yield formation. However, emergence of seed lots with high percentage of abnormal seedlings was longer than for lots with small number of abnormal seedlings. Laboratory germination and germination energy were more correlated with indicators of field emergence rate, while total percentage of germinated seedlings (normal and abnormal) was more correlated with grain yield. However, it can be expected that in adverse environment conditions, abnormal seedlings are less likely to develop into normal plants.
Low temperature has a negative effect on germination parameters and early seedling growth. Seed priming is a beneficial pre-sowing treatment that partially hydrates the seeds to the point of germination process initiation, followed by drying which prevents radicle protrusion. The aim of this study was to evaluate the effect of different seed priming treatments on some maize seed vigor and early seedlings growth parameters at different temperature conditions. Seeds were primed in water (hydropriming) and KNO 3 solution (0,1% and 0,5%) at 25°C for 17 h. Germination was tested at 25°C, 15/25°C and 15°C. Seed priming treatments had more improving effects on studied seed and seedlings traits under suboptimal (15°C and 15/25°C) than at optimal temperature conditions. Seed priming treatment with 0,5% KNO 3 had the most beneficial effects and increased the most of studied parameters under suboptimal conditions. Seed priming with KNO 3 could be used to achieve higher seed vigor and seedling growth at suboptimal temperature conditions in maize.
One of the most important phases in commercial maize breeding programs is the assessment of the value of newly-developed progeny by testing in hybrid combinations. In this study, non-parametric stability measures were applied to analyze the genotype ? environment interaction and to assess phenotypic stability of two half-sib maize populations, each consisting of 40 genotypes, across 9 variable environments. Non-parametric tests of significance determined the presence of qualitative interaction for grain yield in both observed populations. Results of the stability analysis showed no significant differences between the two progeny groups indicating that the used testers did not bring significant increase in stability in either of the analyzed half-sib populations. Individual genotypes were also compared based on grain yield stability within both progeny groups using the stability parameters Si(1), Si(2), Si(3) and Si(6). Association between the grain yield and stability indices Si(1) and Si(2) of the analyzed genotypes was presented graphically enabling the identification of genotypes which can be recommended for further breeding process as the most promising ones. The correlations between grain yield and stability parameters were tested by Spearman?s rank correlations. Both progeny groups (HS1 and HS2) showed no significant correlations between the grain yield and stability parameters Si(1) and Si(2), but the rank correlations between Si(1) and Si(2) values were very strong and highly significant. Highly significant negative correlations were found between grain yield and stability indices Si(3) and Si(6) in both progeny groups, and very strong and highly significant correlations were found between Si(3) and Si(6) values.
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