Bambara Groundnut seeds produced in this season often stored for some period of time until the next planting season. Storage period is one of the factors determine the quality of seeds while seeds was stored. This research conducted to determine the effect of storage period on seed germination. Seed viability and seed vigour were observed in 6 promising lines of Bambara Groundnut on 2 different storage period. Experiments was conducted in Agronomy Department, Faculty of Agriculture, Brawijaya University, Malang Indonesia, from May to June 2022. Six promising lines of Bambara groundnut produced from different seasons were stored in cold storage for one year and two years. Each promising line that was stored on respective years was tested with between paper method (BP) according to ISTA. Data collected from the observation of germination, viability characters, vigour characters. Experiments arranged in completely randomized design with combinations of 2 storage period; 1 year, 2 years and 6 promising lines of Bambara Groundnut; CCC 1.6, PWBG 6, PWBG 5.2.1, SS 2.4.2, BBL 1.1, TVSU 86. Results showed that Storage period affect seed viability and vigour. Longer period of storage decreasing some germination characteristics. Seeds stored after 2 years have lower vigour index, final germination percentage and dry weight of shoot and root. Lowest final germination percentage in 2 years storage recorded in PWBG 5.2.1 (70%). Seeds stored on 1 year storage period have higher vigour index (30%-60%), higher germination rate and higher dry weight of shoot and root.