Background: Due to its deep penetration into the dermis, ultraviolet A (UVA) radiation is considered a primary factor in skin photoageing. The aim of this study is to use a qualitative and quantitative analysis to determine the structural parameters of skin photoageing in mice exposed to UVA radiation, with or without the application of a photoprotective cream. Materials and methods: The experiment consisted of the radiation of female BALBc mice in a solarium by UVA rays, up to total dosages of 7800 J/cm 2 and 12500 J/cm 2 . A total of 78 animals were divided into four experimental and two control groups. All animals were shaved and the animals in two experimental groups were treated with a photoprotective cream half an hour before exposure. The samples of the treated skin were stained with haematoxylin-eosin and Van-Gieson staining methods. All measurements, except for the presence of dyskeratosis, were taken using ImageJ 150i software. Results: In the study, the signs of skin photoageing were more evident in untreated groups of animals. Dyskeratosis was more frequent in both of the untreated groups of animals (p = 0.004 and p = 0.003). The lowest values of epidermal thickness (13.8 ± 2.6 μm and 12.7 ± 2.3 μm) were present in both of the untreated groups of animals (p < 0.001 and p < 0.001). The highest values of stratum corneum thickness (34.3 ± 8.5 μm) were observed in the untreated, shorter radiated group of animals (p < 0.001) which was irradiated for the shortest period of time. Beside the control groups, the highest length of dermo-epidermal junction was recorded in the group of treated, longer radiated animals (1467.6 ± 94.6 μm; p = 0.373). The lowest values of dermal thickness (115.9 ± 10.5 μm and 134.8 ± 21.8 μm) and volumetric density of the collagen fibres (31.92 ± 3.19% and 29.40 ± 4.54%) were present in both untreated groups of animals (p < 0.001, p < 0.001, p = 0.035). Conclusions: Skin photoageing was most pronounced in the groups of animals irradiated without the application of photoprotective cream.

No abstract

Odre ivanje preoperativnih vrednosti serumskog prostata specifi nog antigena (PSA) predstavlja primarnu proceduru u dijagnozi razli itih patoloških promena u prostati (karcinoma prostate-KP, prostati ne intraepitelne neoplazije-PIN i benigne hiperplazije prostate-BHP), nakon ega sledi digitorektalni pregled i biopsija prostate kao zlatni standard. Me utim, visoka senzitivnost i niska specifi nost PSA testa u dijagnozi karcinoma prostate (KP) predstavlja problem u klini koj praksi. Cilj rada je utvrditi dijagnosti ke perfomanse serumskog PSA u dijagnozi KP, PIN i BHP. Ispitivanjem je obuhva eno 100 pacijenata podeljenih u tri grupe: 70 sa KP, 20 sa PIN i 10 sa BHP. Pacijenti sa PIN i BHP inili su kontrolnu grupu. Preoperativne vrednosti PSA odre ene su metodom Tandem-R, a na osnovu dobijenih rezultata pacijenti su podeljeni u grupe: 4-10, 11-20, 21-30, 31-40 i >40. Definitivna patohistološka dijagnoza postavljena je na rutinskim hematoksilin-eozin preparatima. Za svaku vrednost PSA kod KP odre ena je area ispod ROC krivulje, senzitivnost-SE i specifi nost-SP. Preoperativne vrednosti serumskog PSA kod pacijenata sa KP (medijan-35,82 ng/ml, min-6 ng/ml, max-960,40 ng/ml) zna ajno su ve e u odnosu na PIN (medijan-9,15 ng/ml, min-3,16 ng/ml, max-27,61 ng/ml) i BHP (medijan-8,68 ng/ml, min-0,80 ng/ml, max-31,20 ng/ml). Najbolje dijagnosti ke karakteristike PSA pokazuju se pri grani noj vrednosti koncentracije od 10 ng/ml (AUC=0,781; SE=92,9%; SP=63,3%; p<0,0001). Vrednost PSA je od velike pomo i u dijagnostici manifestnog, ali i po etnih formi KP. Sve vrednosti PSA iznad 10 ng/ml svakako treba da pobude zna ajnu sumnju na postojanje karcinoma prostate, pre nego na bilo koju drugu patohistološku promenu. Klju ne re i: karcinom prostate, prostata specifi ni antigen, senzitivnost, specifi nost.

Uvod: Ultravioletno A (UVA) zra enje sunca i vešta kih emitera je najzna ajniji faktor fotostarenja kože. Cilj: Cilj našeg istraživanja bio je da se utvrdi prisustvo promena u izgledu i bojenju kolagenih i elasti nih vlakana derma kože miševa izloženih dejstvu UVA zra enju, sa ili bez upotrebe fotoprotektivne kreme. Metode: Eksperiment se sastojao u zra enju ženki BALBc soja miševa, u solarijumu UVA lampama. Životinje su bile podeljene u dve eksperimentalne i dve kontrolne grupe. Pre izlaganja zra enju životinjama eksperimentalnih i jedne kontrolne grupe je brijanjem, tri puta nedeljno odstranjivana dlaka sa le a, a polovini eksperimentalnih životinja je pola sata pre ekspozicije nanošena fotoprotektivna krema sa širokim spektrom zaštite (UVA filterom i SPF 50). Druga kontrolna grupa nije bila podvrgavana brijanju. Eksperimentalne grupe životinja zra ene su 5 puta nedeljno, po 2 sata dnevno (doza 156 J/cm2), tokom 16 nedelja, do ukupne doze zra enja od 12500 J/cm2. Ovaj deo kože je primenom razli itih histiohemijskih metoda bojenja koriš en za histološka ispitivanja. Rezultati: Prisustvo izraženih znakova fotošte enja kolagenih vlakana je statisti ki zna ajno potvr eno u nemazanoj eksperimentalnoj grupi životinja (p <0,001), po etno fotoošte enje u mazanoj eksperimentalnoj grupi životinja (p <0,001), normalan izgled i bojenje kolagenih vlakana potvr en je kod obe kontrolne grupe životinja (p <0,001). Prisustvo po etnih znakova solarne elastoze zabeleženo je u nemazanoj eksperimentalnoj grupi životinja. Zaklju ak: Rezultati su pokazali da je primena fotoprotektivne kreme sa širokim spektrom zaštite kod polovine eksperimentalnih životinja, opravdala upotrebu i dovela do efikasnog preveniranja ili zna ajnog smanjenja promena na nivou derma kože miševa izloženih dejstvu UVA zra enja. Klju ne re i: derm, kolagena vlakna, elasti na vlakna, BALBc, fotoprotektivna krema

This study examines the efficiency of UVA radiation in the eradication of murine mites. The experiment consisted of the radiation of female BALBc mice in a solarium by UVA rays, up to total dosages of 7800 J/cm2 and 12 500 J/cm2. A total of 88 animals were divided into four experimental and three control groups. Animals from the experimental and two control groups were shaved, and animals in two experimental groups were treated by a photoprotective cream half an hour before exposure. The animals in the third control group were not shaven. The presence of mites was detected in histological preparations of most of the animals in the control groups. When compared to the experimental groups of animals exposed to radiation up to 7800 J/cm2, the presence of mites was significantly more frequent in the control group of shaven animals (p = 0.013) and unshaven animals (p < 0.001). When compared to the experimental groups exposed to radiation up to 12 500 J/cm2, the presence of mites was significantly more frequent in the control group of shaven animals (p < 0.001) and unshaven animals (p < 0.001). Median value of mites was statistically much higher in the control, unshaven group of animals in comparison to the untreated group of animals exposed to radiation up to 7800 J/cm2 (p = 0.006). Median value of mites between untreated group of animals exposed to radiation up to 7800 J/cm2 and their shaven control group was near a statistically significant difference (p = 0.056). By comparing the number of mites between the control groups of animals, no presence of statistically significant difference (p = 0.901) was confirmed. UVA radiation led to the effective eradication of murine mites.