In September of 2017, Hurricane Irma made landfall within the Rookery Bay National Estuarine Research Reserve of southwest Florida (USA) as a category 3 storm with winds in excess of 200 km h−1. We mapped the extent of the hurricane’s impact on coastal land cover with a seasonal time series of satellite imagery. Very high-resolution (i.e., <5 m pixel) satellite imagery has proven effective to map wetland ecosystems, but challenges in data acquisition and storage, algorithm training, and image processing have prevented large-scale and time-series mapping of these data. We describe our approach to address these issues to evaluate Rookery Bay ecosystem damage and recovery using 91 WorldView-2 satellite images collected between 2010 and 2018 mapped using automated techniques and validated with a field campaign. Land cover was classified seasonally at 2 m resolution (i.e., healthy mangrove, degraded mangrove, upland, soil, and water) with an overall accuracy of 82%. Digital change detection methods show that hurricane-related degradation was 17% of mangrove forest (~5 km2). Approximately 35% (1.7 km2) of this loss recovered one year after Hurricane Irma. The approach completed the mapping approximately 200 times faster than existing methods, illustrating the ease with which regional high-resolution mapping may be accomplished efficiently.

The goal of this study was to investigate the effects of light intensity, genotype, and various chemical treatments on chloroplast movement in guard cells of Arabidopsis thaliana leaves. After treatment at various light intensities (dark, low, and high light), leaf discs were fixed with glutaraldehyde, and imaged using confocal laser microscopy. Each chloroplast was assigned a horizontal (close to pore, center, or epidermal side) and vertical (outer, middle, inner) position. White light had a distinct effect on chloroplast positioning, most notably under high light (HL) when chloroplasts on the upper leaf surface of wild-type (WT) moved from epidermal and center positions toward the pore. This was not the case for phot1-5/phot2-1 or phot2-1 plants, thus phototropins are essential for chloroplast positioning in guard cells. In npq1-2 mutants, fewer chloroplasts moved to the pore position under HL than in WT plants, indicating that white light can affect chloroplast positioning also in a zeaxanthin-dependent way. Cytochalasin B inhibited the movement of chloroplasts to the pore under HL, while oryzalin did not, supporting the idea that actin plays a role in the movement. The movement along actin cables is dependent on CHUP1 since chloroplast positioning in chup1 was significantly altered. Abscisic acid (ABA) caused most chloroplasts in WT and phot1-5/phot2-1 to be localized in the center, middle part of the guard cells irrespective of light treatment. This indicates that not only light but also water stress influences chloroplast positioning.

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Recently, strong top-down (consumer) control of cordgrass (Spartina alterniflora) has been demonstrated. Here, we manipulated the densities of cordgrass consumers, acridid grasshoppers (Melanoplus bivittatus and Melanoplus femurrubrum), to examine their impact on cordgrass in the Plum Island Estuary (PIE), MA, USA. After 1 month, there was no detectable effect of grasshopper density on S. alterniflora biomass and grasshoppers at the highest densities (34 individuals per square meter) consumed onlỹ 14% of the standing stock biomass. However, significant impacts of grasshopper density on grazing damage were seen. For example, plant damage and scarring length increased by 160% and 6,156%, respectively, at the highest grasshopper densities relative to exclusion (zero grasshoppers) densities. Plant height was significantly reduced with increasing grasshopper densities, although this may be a function of leaf tip removal instead of reduced plant growth. No other strong consumers of cordgrass (e.g., Littoraria irrorata, Prokelisia marginata) were observed in PIE and we suggest that consumer regulation of cordgrass is weak in this system.

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