The HET-CAM (Hen"s Egg Chorioallantoic Membrane) assay is a qualitative alternative method to the in vivo Draize Rabbit Eye test to assess the irritancy potential of chemicals. In this work, for the first time the ophthalmic irritation of 13 different protic ionic liquids has been evaluated using ImageJ and PhotoSkape image processing programs to analyze the results of the HET-CAM assay. The irritation potential of a substance can be quantified using these computational tools by observing blood vessel changes like lysis, hemorrhage and coagulation. In conclusion, the modification of the established HET-CAM assay made it possible to determine the damage to minute blood vessels, highlighting the low-irritant profile of some studied protic ionic liquids to the ocular tissues.