-The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2-21, with an average of 8.63. The accessions exhibit a significant number of rare alleles, with genotypes 19, 35, 63 and 65 carrying the greater number of exclusive alleles. Accessions 75 and 79 were the most similar and accessions 31 and 35, and 40 and 78, were the most divergent ones. A low correlation between SSR and EST-SSR data was observed, thus genomic and expressed microsatellite markers are required for an appropriate analysis of genetic diversity in soybean. The genetic diversity observed was high and allowed the formation of five groups and several subgroups. A moderate relationship between genetic divergence and geographic origin of accessions was observed.Index terms: Glycine max, molecular marker, plant breeding, plant germplasm, polymorphism information content. Termos para indexação: Glycine max, marcadores moleculares, melhoramento de plantas, germoplasma vegetal, conteúdo de informação polimórfica. Diversidade genética em germoplasma de soja identificada por marcadores SSR e EST-SSR
The Brazilian Northeastern dry forest (Caatinga) is one of the diversification centers of Mimosa species. We determined the characteristics of native rhizobia isolates from nodules of Mimosa tenuiflora and Mimosa paraibana grown in pots with soils collected under Caatinga vegetation and compared the restriction ribosomal DNA profiles of the isolates with those of 16 reference strains. All plants formed abundant indeterminate nodules and all nodule isolates formed fast growing colonies. No colony altered the medium to an alkaline reaction and most of them produced low or medium amounts of extracellular polysaccharides. White and creamy colonies predominated among the isolates but orange and green colonies were present. Differences among the isolates from the Mimosa species tested are indicated by the greater phenotypic diversity of those obtained from M. tenuiflora. The analysis of the 16S rDNA gene suggests that the isolates from M. tenuiflora and M. paraibana are closely related and closer to -rhizobia than to α-rhizobia. However, the similarity with all the tested -rhizobia reference strains was relatively low suggesting that the isolates may belong to different bacteria species.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.