In patients with CHF and inspiratory muscle weakness, inspiratory muscle loading results in marked reduction of blood flow to resting and exercising limbs. Inspiratory muscle training improves limb blood flow under inspiratory loading in these patients.

As análises da concentração sanguínea de lactato e das trocas gasosas respiratórias são métodos tradicionalmente empregados para identificar a transição de produção de energia pelo metabolismo muscular. No entanto, mais recentemente, vem sendo sugerido método alternativo mediante análise da variabilidade da freqüência cardíaca. Pretendeu-se, com o presente estudo, estabelecer comparações entre o limiar de variabilidade da freqüência cardíaca (LiVFC) e o primeiro limiar ventilatório (LV1), em uma amostra de adolescentes. Para tanto, foram submetidos a teste de esforço físico de carga máxima em esteira ergométrica 41 sujeitos (22 rapazes e 19 moças) com idades entre 15 e 18 anos. O LV1 foi identificado mediante o equivalente ventilatório de oxigênio envolvendo recursos de ergoespirometria. A variabilidade da freqüência cardíaca foi analisada por intermédio dos intervalos R-R, através da plotagem de Poincaré, que oferece informações quanto ao desvio-padrão da variabilidade instantânea batimento-a-batimento (SD1), ao desvio-padrão a longo prazo de intervalos R-R contínuos (SD2) e à razão SD1/SD2. O LiVFC foi identificado pelo SD1 de acordo com três critérios: (1) diferenças entre o SD1 de dois estágios consecutivos menor que 1ms; (2) SD1 menor que 3ms; e (3) ocorrência de ambos os critérios em conjunto. Mediante análise dos resultados verificou-se que os intervalos R-R e SD2 diminuíram progressivamente a cada intervalo de 10% do VO2pico até o final do teste de esforço físico (0,05 < p < 0,01). O SD1 diminuiu significativamente desde 20% até 50% do VO2pico. A partir de 60% até o VO2pico o SD1 não apresentou diferenças significativas. A razão SD1/SD2 aumentou a partir de 60%. O LV1 ocorreu a 54,4 ± 8,8% do VO2pico enquanto o LiVFC, a 52,4 ± 12,5%, 57,0 ± 14,1% e 57,8 ± 13,8% do VO2 pico, para os critérios 1, 2 e 3, respectivamente. Não foram observadas diferenças estatísticas entre o LV1 e os três critérios utilizados para identificação do LiVFC. Observaram-se coeficientes de correlação momento-produto significativos entre o LiVFC identificado mediante os três critérios considerados e o LV1, quando foram utilizados os valores absolutos de VO2. Porém, não foram encontradas correlações estatísticas significativas entre o LiVFC e a identificação do LV1 expresso em proporção do VO2pico. Em assim sendo, concluiu-se que parece ser precipitado tentar empregar o LiVFC como método alternativo na identificação do LV1 de adolescentes.

Heat therapy has been shown to promote capillary growth in skeletal muscle and in the heart in several animal models, but the effects of this therapy on angiogenic signaling in humans are unknown. We evaluated the acute effect of lower body heating (LBH) and unilateral thigh heating (TH) on the expression of angiogenic regulators and heat shock proteins (HSPs) in healthy young individuals. Exposure to LBH ( n = 18) increased core temperature (Tc) from 36.9 ± 0.1 to 37.4 ± 0.1°C ( P < 0.01) and average leg skin temperature (Tleg) from 33.1 ± 0.1 to 39.6 ± 0.1°C ( P < 0.01), but did not alter the levels of circulating angiogenic cytokines and bone marrow-derived proangiogenic cells (CD34+CD133+). In skeletal muscle, the change in mRNA expression from baseline of vascular endothelial growth factor (VEGF), angiopoietin 2 (ANGPT2), chemokines CCL2 and CX3CL1, platelet factor-4 (PF4), and several members of the HSP family was higher 30 min after the intervention in the individuals exposed to LBH ( n = 11) compared with the control group ( n = 12). LBH also reduced the expression of transcription factor FOXO1 ( P = 0.03). Exposure to TH ( n = 14) increased Tleg from 32.8 ± 0.2 to 40.3 ± 0.1°C ( P < 0.05) but Tc remained unaltered (36.8 ± 0.1°C at baseline and 36.9 ± 0.1°C at 90 min). This intervention upregulated the expression of VEGF, ANGPT1, ANGPT2, CCL2, and HSPs in skeletal muscle but did not affect the levels of CX3CL1, FOXO-1, and PF4. These findings suggest that both LBH and TH increase the expression of factors associated with capillary growth in human skeletal muscle.

Voluntary wheel running (RUN) prevents declines in insulin-mediated vasodilation, an important component of insulin-mediated glucose disposal, in rats prone to obesity and insulin resistance. Objective Determine whether RUN: 1) improves insulin-stimulated vasodilation after insulin resistance has been established, and 2) differentially affects arterioles from red and white muscle. Methods Insulin signaling and vasoreactivity to insulin (1–1000 μIU/mL), were assessed in second order arterioles (2A) from the white (Gw) and red (Gr) gastrocnemius of sedentary OLETF rats at 12 and 20 weeks of age (SED12; SED20) and those undergoing RUN (RUN20) or caloric restriction (CR20; to match body weight of RUN) from 12–20 weeks. Results Glucose and insulin responses to i.p. glucose were reduced in RUN20, elevated in SED20 (P<0.05 vs. SED12), and maintained in CR20. Insulin-stimulated vasodilation was greater in Gw, but not Gr, 2As of RUN20 (P<0.01 vs. all groups) and was improved by ET-1 receptor inhibition in Gw 2As from SED20 and CR20 (P<0.05). There were no differences in microvascular insulin signaling among groups or muscle beds. Conclusions RUN selectively improved insulin-mediated vasodilation in Gw 2As, in part through attenuated ET-1 sensitivity/production, an adaptation that was independent of changes in adiposity and may contribute to enhanced insulin-stimulated glucose disposal.

Leg thermotherapy (TT) application reduces blood pressure (BP) and increases both limb blood flow and circulating levels of anti-inflammatory mediators in healthy, young humans and animals. The purpose of the present study was to determine the impact of TT application using a water-circulating garment on leg and systemic hemodynamics and on the concentrations of circulating cytokines and vasoactive mediators in patients with symptomatic peripheral artery disease (PAD). Sixteen patients with PAD and intermittent claudication (age: 63 ± 9 yr) completed three experimental sessions in a randomized order: TT, control intervention, and one exercise testing session. The garment was perfused with 48°C water for 90 min in the TT session and with 33°C water in the control intervention. A subset of 10 patients also underwent a protocol for the measurement of blood flow in the popliteal artery during 90 min of TT using phase-contrast MRI. Compared with the control intervention, TT promoted a significant reduction in systolic (∼11 mmHg) and diastolic (∼6 mmHg) BP (P < 0.05) that persisted for nearly 2 h after the end of the treatment. The serum concentration of endothelin-1 (ET-1) was significantly lower 30 min after exposure to TT (Control: 2.3 ± 0.1 vs. TT: 1.9 ± 0.09 pg/ml, P = 0.026). In addition, TT induced a marked increase in peak blood flow velocity (∼68%), average velocity (∼76%), and average blood flow (∼102%) in the popliteal artery (P < 0.01). These findings indicate that TT is a practical and effective strategy to reduce BP and circulating ET-1 concentration and enhance leg blood flow in patients with PAD.