Video tracking systems have been used widely to analyze Drosophila melanogaster movement and detect various abnormalities in locomotive behavior. While these systems can provide a wealth of behavioral information, the cost and complexity of these systems can be prohibitive for many labs. We have developed a low-cost assay for measuring locomotive behavior and seizure movement in D. melanogaster. The system uses a web-cam to capture images that can be processed using a combination of inexpensive and free software to track the distance moved, the average velocity of movement and the duration of movement during a specified time-span. To demonstrate the utility of this system, we examined a group of D. melanogaster mutants, the Bang-sensitive (BS) paralytics, which are 3-10 times more susceptible to seizure-like activity (SLA) than wild type flies. Using this novel system, we were able to detect that the BS mutant bang senseless (bss) exhibits lower levels of exploratory locomotion in a novel environment than wild type flies. In addition, the system was used to identify that the drug metformin, which is commonly used to treat type II diabetes, reduces the intensity of SLA in the BS mutants.

Mid-spatial-frequency surface errors can be introduced by various manufacturing processes. These errors bridge the gap between traditional figure and finish errors. Although the effects of mid-spatial-frequency errors on the imagery of an optical system can be modeled with a ray-based approach, simply tracing rays provides little insight. We present an alternative method that treats surface errors as perturbations to the nominal surface profile. This approach, combined with standard statistical methods, allows one to make simple back-of-the-envelope predictions of the effects of mid-spatial-frequency errors for various measures of optical performance. Two examples illustrating the effectiveness of this approach are presented.

No abstract

We investigate unobstructed, plane-symmetric imaging systems of four spherical mirrors. Fifteen parameters are necessary to specify the configuration of such a system. Constraints are determined that ensure that any resultant system possesses a given set of first-order properties. These constraints remove four parameters as available degrees of freedom. To illustrate the efficacy of this design approach, we present two example studies: one for a class of systems with the object at infinity and another for finite-conjugate projection systems. For each study a global optimizer is used as the primary search tool. Example systems from these studies are presented.

No abstract