Mycobacterium tuberculosis is infectious bacteria and causes tuberculosis in humans. M. tuberculosis infects the immune deficient human and shows the symptoms of the infection. Also bacteria stay in latent phase inside the human body and can be active in suitable conditions. One third of the total population of the world is infected by M. tuberculosis; therefore it is very important to have potential drugs against tuberculosis. Mycobacteria have reported multidrug resistance to the available drugs for tuberculosis. Hence, there is a need to find a new target for the drugs. Fatty acid synthase II (FAS II) is the enzyme that catalyzes the synthesis of fatty acid and is not found in humans. It is a multifunctional polypeptide, composed of different domains in which can be targeted individually to inhibit the function of FAS II. 2-Nitropropane Dioxygenase is a part of enoyl reductase domain in FAS II and can be potentially targeted. In this study, the homology modeling of 2NPD from M. tuberculosis has been done and small molecules that have the potential to bind and inhibit the function of the enzyme have been identified. Also the stability of proteinligand complex was determined.

Background: The extended spectrum beta lactamases producing bacteria are bacteria of great concern among Gram negative bacilli. Escherichia coli stand out as major carrier of this enzyme. The appropriate control of this resistance pattern depends on using the antimicrobial regimen of best choice. Therefore the value of the susceptibility profile of organism harboring this enzyme cannot be overemphasized. Objectives: To determine the antimicrobial susceptibility of extended spectrum beta lactamases (ESBL) producing and the non-ESBL producing strains of Escherichia coli from clinical isolates of Escherichia coli in University of Maiduguri Teaching Hospital. Methodology: Confirmed variants of Escherichia coli were screened and confirmed for ESBL possession. Subsequently, modified Kirby Bauer method was utilized to test for antibiotic susceptibility using the commercially available Oxoid single disc for some major antibiotics. Results: A total of 172 strains of Escherichia coli were identified during the study period. Out of this number; 131 were identified as ESBL positive while a total of 41 were ESBL negative. The highest sensitivity for both the ESBL positive and ESBL negative strains of Escherichia coli was observed with Imipenem followed closely by Gentamicin. Conclusion: The study reveals narrow choice of antibiotics for the ESBL positive isolates of Escherichia coli although Imipenem antibiotic still retains its sensitivity.