Potential waste of local crab carapace (Portunus pelagicus) as a source of chitosan as an active layer that can protect bananas has been studied. The process in this study consists of three stages. The first stage was the isolation of chitin through deproteinization process using 2.0 N NaOH solution with a ratio of 1:6 w/v and demineralization process using 1.5 N HCl solution with a ratio of 1:12 w/v. The second stage is the deacetylation stage using 50% NaOH solution with a ratio of 1:20 w/v. Fourier Transform Infra-Red (FTIR) Spectroscopy is used to determine the degree of deacetylation. The third stage is the banana coating application using chitin solution to determine the shelf life of bananas with variations in levels of 2, 2.5, 3 and 3,5 % w/v by immersion method for one hour. It was found that carapace crab, a part that was underutilized from crab, gave rise to chitin deacetylation with a deacetylation rate of 62.11%; pH 8.9 and water content of 7.677%. Chitosan-based coatings are applied to fresh bananas and are found to increase fruit firmness, and inhibit browning. The results show that chitosan-coated bananas have a longer storage time. The application of chitin deacetylated (chitosan) as fruit banana coater found that higher coater levels extend the shelf life of bananas with the best coater content is 3% b/v. It results in a shelf life of bananas for up to 12 days, this is longer than bananas without chitosan layer which only has a shelf life of four days. Increased coating rates have a positive effect on the shelf life of bananas. This study shows that waste from carapace crabs can be used to form active layers that can preserve fruit.

The technology for extracting chitin from shell and other materials needs to be continuously improved, including its conversion to chitosan. Chitosan is a biocompatible polymer, biodegradable, non-toxic, water-soluble at pH below 6.5, and it has protonated amino groups. The benefits of chitosan in industry, food and medicine make it necessary to fully study an efficient chitosan synthesis method and the results can be applied on an industrial scale. This study examined the effect of ultrasonic-assisted in increasing the degree of deacetylation of chitosan produced from Portunus pelagicus shell waste. The production process of chitosan goes through the stages of deproteination, demineralization and deacetylation. All these steps are ultrasound assisted processes with a frequency of 40 kHz through a digital ultrasonic cleaner. Ultrasonic-assisted chitin and chitosan were examined using FTIR spectrometry. The results showed that the ultrasonic method was able to increase the deacetylation degree of chitin with a value of 68.45±0.11% compared to 62.52±0.08% without ultrasonic. Application of ultrasonic assisted deacetylation gave a deacetylation degree of 85.35 ± 0.20%, higher than without ultrasonic 80.24 ± 0.19%.  Physically, ultrasonic-assisted chitosan is smoother and brighter in color. The ultrasonic-assisted chitosan manufacturing method could increase the deacetylation degree and produce high grade chitosan.

Kedawung (Parkia biglobosa) contains various ingredients such as alkaloids, saponins, tannins, flavonoids, and terpenoids. The flavonoid content in Kedawung is thought to have an antioxidant effect. Antioxidants are able to counteract free radicals that enter the body by donating electrons and binding them. Currently, the microwave-assisted extraction (MAE) method is widely used because the solute mass transfer from the sample matrix into the solvent is higher than the Soxhlet method. The following study aimed to know the effect of solvent ratio and extraction time on the extraction yield, flavonoid concentration, and antioxidant activity of kedawung leaf through microwave-assisted extraction. In this method, we used 40% ethanol to make the varied solute: solvent ratio such as 1:20, 1:30, 1:40, and 1:50. The extraction time used in this method was 4-7 minutes respectively. Microwave-assisted extraction has good performance to extract the active substance in Kedawung leaves. The highest yield 16.36%, total flavonoid content (57.32±2,2 mg QE/g extract), and DPPH scavenging activity (88.87±1.062%) was obtained in the extraction with a solids-solvent ratio of 1:40 g/mL, at an extraction time of 6 minutes. This method promises to take the active substance in a short time.

Background: Morinda citrifolia L. is widely used as traditional medicine for various diseases. The benefits of noni are studied from the seeds, fruit, leaves and root bark. This leaf active compound is rich in flavonoids, so an effective extraction process is needed to extract it. Conventional extraction generally takes a long time and involves a thermal process that can damage the compound, so it requires extraction with the latest methods, one of which is the use of ultrasonic waves. Aim: This study aims to examine the effect of variations in extraction process conditions on present yield, DPPH scavenging activity, flavonoid content and phenol content of Morinda citrifolia L leaves by varying the solids-solvent ratio (1:10, 1:20, 1:30, dan 1:40 g/mL), and extraction temperature (25, 35, 45, and 55ºC). Method: The process uses the ultrasonic assisted extraction method with 50 %V ethanol for 60 minutes. Result: The highest yield was obtained in the extraction with a solids-solvent ratio of 1:40 g/mL, at an extraction temperature of 55ºC, which was 32.29±0.066%. The highest flavonoid content (173.41±0.615 mg quercetin equivalent/g extract), phenol content (197.00±0.148 mg gallic acid equivalents/g extract) and DPPH scavenging activity (97.65±0.912%) was obtained in the extraction with a solids-solvent ratio of 1:30 g/mL, at an extraction temperature of 25ºC. The best extract measured antioxidant activity and IC50 values obtained with 23.21 µg/mL. Conclusion: The use of the ultrasonic assisted extraction method by selecting the optimal operating conditions greatly increases the amount of active compound uptake required.