Today, business styles and ways of doing business are changing due to global and technological changes. Employees' attitudes towards these changes are important for employers. At the point of business life; with the increase of working parents, the importance of the balance between private life and work-life is also increasing. The ability of employees to establish a work-life balance makes their lives easier. One of the practices used by organizations to maintain the work-life balance of employees is flexible working practices. Employees' attitudes towards flexible working may vary within the framework of their perceptions, needs, and expectations. The aim of this study is to investigate the effect of employees' attitudes towards work-life balance and flexible working practices on their motivation levels. In this context, a questionnaire was applied to 543 people working in the central units of Ankara Metropolitan Municipality and its subsidiary EGO General Directorate. IBM SPSS Statistics 25 program was used in the analysis of the data in the research. The reliability of the data used in the questionnaire was tested and factor analysis was performed. Correlation analysis to determine the existence of the relationship between independent and dependent variables; Regression analysis was performed to measure the interaction. In the study, Anova and T-tests were applied to examine the difference between variables and demographic variables. In the research, between the dimensions of work-life balance and motivation, intrinsic motivation and extrinsic motivation; Significant differences were found between the sub-dimensions of work-life balance, work responsibility, and other employee attitudes of the attitude variable towards flexible working arrangements and the intrinsic motivation and extrinsic motivation dimensions of the motivation variable.