Fresh-cut leafy vegetable has an image as a healthy, nutritious, and delicious product. However, the product still faces the challenge of quality retention and short shelf life, especially in tropical climate regions. Enzymatic browning in fresh-cut leafy vegetables is considered one of the most important attributes limiting the shelf life of the product. The hurdle approach using commercial vacuum precooling in combination with modified atmosphere packaging (MAP) as an alternative to the use of chemical preservatives to prevent enzymatic browning, an undesirable attribute that is easily detected by consumers, was investigated. The hurdle technology exhibited synergistic effects on fresh-cut lettuce, namely frillice iceberg, romaine, and red oak, in slowing down cut-surface browning, maintaining quality, delaying microbial growth, and extending shelf life of salad products at the retail level. The findings of the study verified the potential of the hurdle approach in delaying the effect of cutting as well as extending shelf life of the product stored at 4 ± 1 °C with 85% RH from three days to nine days with an additional unit cost of 1.05%. Therefore, our hurdle approach is anticipated as the practice with non-chemical and economical approach in the supply chain of the fresh-cut, leafy vegetables industry.

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An effect of breaking the cold chain on cos lettuce (Lactuca sativa var. longifolia) had been studied in this research. Cos lettuce was taken under cold chain system at 4°C. Imitating the exposure to high temperature in hot country, cos lettuce was put out in the 25, 30, 35,and 40C atmosphere for 15, 30, 45 and 60 min. Product life, weight loss percentage, change of colour and chlorophyll content were measured to investigate quality degradation of cos lettuce. The limitation and good practices cos lettuce which had to go through cold chain breakage were acquired. From the results, it is recommended that cos lettuce should not exposed to temperature higher than 25C for longer than 30 min in order to maintain acceptable quality and freshness close to absolute cold chain, considered from degradation of weight, colour and chlorophyll. Degree of temperature had influences on degrading levels of cos lettuce. Exposing to temperature higher than 35C would degrade its quality and freshness and reduce storage life and should be avoid in any cases.

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