No abstract

Background Nephrotic syndrome is one of the common cause among the kidney disease in children worldwide .It is characterised by the edema proteinuria, hypoalbuminemia and hypocholestromia. Recently many studies have emerged with the association of microRNAs playing an potential roles in many pathophysiological functions. MicroRNAs(miRNAs ) and RNA binding proteins (RBPs) are found to be two most important needed transcriptional regulators of gene expression as well as for the aberrant expression that tend to contribute to the development of the disease. It can reduce translation neither by translation repression through or MicroRNA cleavage. In this present study we have checked for the expression pattern of the targeted microRNAs miR-17-5P,miR-155p ,miR- 424 -5p ,miR-1 and 215-5p in the Children among Steroid Sensitive Nephrotic syndrome(SSNS) Steroid Resistance Nephrotic Syndrome (SRNS)along with the healthy individuals. Total RNA was isolated from the urine samples among the three groups(SSNS = 100,SRNS = 100 and healthy individuals100).The expression pattern for theses microRNAs was carried out using RT-PCR .Bioinformatics tools such as miRWalk, miR-Tar link were used in predicting targets for the microRNAs an online data bases and g profiler software is used which was also helpful in evaluating the targets based on the biological functions, Molecular functions and the pathways related to the selected microRNAs, along with that ROC analysis was also performed which was widely helpful in selecting the microRNAs that could be used as a potential biomarker as well as a therapeutic target. Among the five microRNAs miR- 1,miR-215, miR-17-5P, ,miR-155-5p & miR-424-5p.Two microRNAs miR-424-5p& miR-155p is found to be up regulated in the SRNS group.

Background Recently, urinary exosomal miRNAs are gaining increasing attention as their expression profiles are often associated with specific diseases and they exhibit great potential as noninvasive biomarkers for the diagnosis of various diseases. The present study was aimed to evaluate the expression status of selected miRNAs (miR-1, miR-215-5p, miR-335-5p and let-7a-5p) in urine samples from children with NS [steroid sensitive (SSNS)] and [steroid resistant (SRNS)] along with healthy control group.Methods MicroRNA isolation was carried out in urine samples collected from SSNS (100 nos), SRNS (100 nos), and healthy controls (50 nos) using MiRNeasy Mini Kit, followed by cDNA conversion for all the four selected miRNAs using Taqman advanced miRNA cDNA synthesis kit and their expression was quantified by Taqman Advanced miRNA assay kits using Real Time PCR Machine and Rotogen-Q in SSNS and SRNS patients and healthy control subjects.ResultsQuantification of all the four miRNAs (miR-1, mir-215, miR-335, let 7a) were found to be upregulated in both SSNS and SRNS as compared to control group. Further, the comparison of microRNAs within the case groups revealed significant downregulation of three microRNAs - miR-1, miR-215, miR- 335 and upregulation of let-7a in SRNS group as compared to SSNS. The t-test performed for all the four miRNAs was found to be statistically significant. ConclusionsThe aberrant expression of all the four microRNAs in both SSNS and SRNS as compared to healthy subjects may serve as novel biomarkers to distinguish between NS and healthy controls. The differential expression of microRNA let-7a is useful to discriminate SSNS and SRNS.