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" As part of the Remote Exploration and Experimentation Project (REE), work was performed to do a proton SEE evaluation of the Myricom network protocol system (Myrinet). This testing included the evaluation of the Myrinet crossbar switch and the Network Interface Card (NIC). To this end, two crossbar switch devices and five components in the NIC were exposed to the proton beam at the University of California at Davis Crocker Nuclear Laboratory (CNL).

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Multi-color panel design can be difficult due to the many factors which impact signal detection including the fluorescence detection capability of the cytometer, target molecule density and the spectral characteristics of available fluorophores (fluors). We demonstrate that a flow cytometer with a fixed and standardized fluorescence measurement system simplifies design of multi-color flow experiments, using a common conundrum researchers face as illustration: several different fluor conjugates detectable by the cytometer are available for a given clone. Which is best to use? First we show that by locking down optical alignment and optimizing fluorescence detection at the time of manufacture the BD Accuri C6 gives reproducible, predictable fluorescence measurements both for individual instruments over time and between instruments. We also show that this design leads to predictable fluorescence spillover for stable fluors. Since fluorescence spillover due to bright fluors and/or highly expressed epitopes can mask dimmer signals in neighboring channels, successfully predicting spillover greatly simplifies multi-color panel design. We illustrate this by comparing the spillover of four fluors optimally detected in FL3 of the BD Accuri™ C6 (PerCP, PerCP-Cy™5.5, PE-Cy7, BD Horizon™ PE-CF594), when conjugated to antibody clones of a high (CD45) and low (CD19) epitope-number antigen. We also quantitate the impact of the spillover on detection of weak signals in neighboring channels.